Physical Organic Chemistry Laboratory, Institute of Chemistry, Universidade Estadual de Campinas, UNICAMP, PO Box 6154, Campinas 13083-970, São Paulo, Brazil.
Spectrometry, Sample Preparation and Mechanization Group, Institute of Chemistry, Universidade Estadual de Campinas, UNICAMP, PO Box 6154, Campinas 13083-970, São Paulo, Brazil.
J Agric Food Chem. 2024 May 29;72(21):12281-12294. doi: 10.1021/acs.jafc.4c00756. Epub 2024 May 15.
This study investigated the effect of AgNPs and AgNO, at concentrations equivalent, on the production of primary and secondary metabolites on transgenic soybean plants through an NMR-based metabolomics. The plants were cultivated in a germination chamber following three different treatments: T0 (addition of water), T1 (addition of AgNPs), and T2 (addition of AgNO). Physiological characteristics, anatomical analyses through microscopic structures, and metabolic profile studies were carried out to establish the effect of abiotic stress on these parameters in soybean plants. Analysis of the H NMR spectra revealed the presence of amino acids, organic acids, sugars, and polyphenols. The metabolic profiles of plants with AgNP and AgNO were qualitatively similar to the metabolic profile of the control group, suggesting that the application of silver does not affect secondary metabolites. From the PCA, it was possible to differentiate the three treatments applied, mainly based on the content of fatty acids, pinitol, choline, and betaine.
本研究通过基于 NMR 的代谢组学方法，考察了浓度相当的 AgNPs 和 AgNO3 对转基因大豆植株初生和次生代谢产物生成的影响。这些植物在发芽室内经过三种不同处理进行培养：T0（添加水）、T1（添加 AgNPs）和 T2（添加 AgNO3）。进行了生理特征、通过微观结构进行的解剖学分析以及代谢物谱研究，以确定非生物胁迫对大豆植株这些参数的影响。对 1H NMR 光谱的分析表明存在氨基酸、有机酸、糖和多酚。AgNP 和 AgNO 处理的植物的代谢物谱与对照组的代谢物谱定性相似，表明银的应用并不影响次生代谢物。通过 PCA，可以区分所施加的三种处理，主要基于脂肪酸、肌醇、胆碱和甜菜碱的含量。
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