利用聚（单宁酸）纳米粒子通过荧光和比色法进行亚硝酸盐的可视化检测的简单方法。
Simple method for visual detection of nitrite using fluorescence and colorimetry by poly (tannic acid) nanoparticles.
机构信息
Department of Chemistry and Chemical Engineering, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Department of Chemistry and Chemical Engineering, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
出版信息
Anal Chim Acta. 2023 Jul 4;1263:341280. doi: 10.1016/j.aca.2023.341280. Epub 2023 Apr 25.
The nitration reaction of nitrite and phenolic substances was first used to identify and detect NO by taking fluorescent poly (tannic acid) nanoparticles (FPTA NPs) as sensing platform. With the low cost, good biodegradable and convenient water-soluble FPTA NPs, a fluorescent and colorimetric dual modes detecting assay was realized. In fluorescent mode, the linear detection range of NO was 0-36 μM, the LOD was as low as 3.03 nM, and the response time was 90 s. In colorimetric mode, the linear detection range of NO was 0-46 μM, and the LOD was as low as 27 nM. Besides, a smartphone with FPTA NPs@ agarose hydrogel formed a portable detection platform to test the fluorescent and visible color changes of FPTA NPs for NO sensing as well as for accurate visualization and quantitative detection of NO in actual water and food samples.
亚硝酸盐与酚类物质的硝化反应首先被用于识别和检测 NO，其使用的传感平台是具有荧光的聚（没食子酸）纳米粒子（FPTA NPs）。由于 FPTA NPs 具有低成本、良好的生物降解性和水溶性等优点，因此实现了荧光和比色双模式检测分析。在荧光模式下，NO 的线性检测范围为 0-36 μM，LOD 低至 3.03 nM，响应时间为 90 s。在比色模式下，NO 的线性检测范围为 0-46 μM，LOD 低至 27 nM。此外，智能手机与 FPTA NPs@琼脂糖水凝胶形成了一个便携式检测平台，用于检测 FPTA NPs 的荧光和可见颜色变化，从而对 NO 进行传感，并对实际水样和食物样品中的 NO 进行准确可视化和定量检测。
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