3-羟基巴豆酰基辅酶 A 脱氢酶（Hcd1）的晶体结构来自产琥珀酸丝状杆菌 SB，分辨率为 1.78 Å。
Crystal structure of a putative 3-hydroxypimelyl-CoA dehydrogenase, Hcd1, from Syntrophus aciditrophicus strain SB at 1.78 Å resolution.
机构信息
Department of Microbiology and Plant Biology, University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019, USA.
Price Family Foundation Institute of Structural Biology, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019, USA.
出版信息
Acta Crystallogr F Struct Biol Commun. 2023 Jun 1;79(Pt 6):151-158. doi: 10.1107/S2053230X23004399. Epub 2023 May 25.
Abstract
Syntrophus aciditrophicus strain SB is a model syntroph that degrades benzoate and alicyclic acids. The structure of a putative 3-hydroxypimelyl-CoA dehydrogenase from S. aciditrophicus strain SB (SaHcd1) was resolved at 1.78 Å resolution. SaHcd1 contains sequence motifs and structural features that belong to the short-chain dehydrogenase/reductase (SDR) family of NADPH-dependent oxidoreductases. SaHcd1 is proposed to concomitantly reduce NAD or NADP to NADH or NADPH, respectively, while converting 3-hydroxypimelyl-CoA to 3-oxopimeyl-CoA. Further enzymatic studies are needed to confirm the function of SaHcd1.
摘要
产琥珀酸拟杆菌 SB 菌株是一种模型共代谢菌，可降解苯甲酸和脂环酸。产琥珀酸拟杆菌 SB 菌株（SaHcd1）中一种假定的 3-羟基丙二酰辅酶 A 脱氢酶的结构已在 1.78 Å 分辨率下解析。SaHcd1 包含属于 NADPH 依赖性氧化还原酶短链脱氢酶/还原酶 (SDR) 家族的序列基序和结构特征。SaHcd1 被提议同时将 NAD 或 NADP 还原为 NADH 或 NADPH，同时将 3-羟基丙二酰辅酶 A 转化为 3-氧代丙二酰辅酶 A。需要进一步的酶学研究来确认 SaHcd1 的功能。
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