College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao, 266590, China; State Key Laboratory of Mining Disaster Prevention and Control Co-founded by Shandong Province and the Ministry of Science and Technology, Shandong University of Science and Technology, Qingdao, 266590, China.
Dust Research Branch Institute, Chongqing Research Institute of China Coal Technology and Engineering Group, Chongqing, 400039, China.
J Environ Manage. 2023 Oct 1;343:118181. doi: 10.1016/j.jenvman.2023.118181. Epub 2023 May 23.
With the recent increases in energy demands, the dust hazards of coal mining caused by transportation, loading and unloading and other processes are becoming increasingly serious. To control dust in open pit coal mines more environmentally friendly and efficiently, and to promote the use and development of non-in situ high-yield urease microorganisms for dust suppression in coal mines, Bacillus pasteurii was selected for dust suppression experiments in this article. Additionally, the growth of microorganisms in the coal dust microenvironment was simulated, and the effect of microbial mineralization products on the calorific value of upper coal dust was further studied. Our findings indicated that Bacillus pasteurii induced dust suppression by forming a calcite precipitate with non-uniform particle size to coal dust cementation. Moreover, after a single spray, the wind erosion resistance efficiency was 84% when the wind speed was set at 10 m/s. The growth of microorganisms and urease activity in the coal dust leachate were largely equal to those in the control group, reaching a peak at approximately 24 h, that the maximum growth quantity of OD was about 1.5, and the maximum urease activity was 11 mmol·L·min. The difference between the peak heat release rate of mixed coal dust and pure coal was only 4.82 kW/m, which would not affect the value of coal products. Non in-situ Bacillus pasteurii can be growth metabolized normally in the microenvironment of coal dust. Finally, the mechanism of coal dust suppression by mineralization of microbial bacterial solution to form calcium carbonate was described by a reaction equation, which is important for further application and development of microbial dust suppressants.
随着能源需求的最近增加，运输、装卸等过程中引起的煤矿粉尘危害越来越严重。为了更环保、更有效地控制露天煤矿的粉尘，促进非原位高产脲酶微生物在煤矿防尘中的应用和发展，本文选择了巴氏芽孢杆菌进行粉尘抑制实验。此外，还模拟了微生物在煤粉微环境中的生长情况，并进一步研究了微生物矿化产物对上层煤粉热值的影响。研究结果表明，巴氏芽孢杆菌通过与煤粉形成大小不一的方解石沉淀来抑制扬尘。而且，当风速设定为 10 m/s 时，单次喷雾的风蚀阻力效率可达 84%。煤粉浸出液中微生物的生长和脲酶活性与对照组基本相当，在 24 h 左右达到峰值，OD 的最大生长量约为 1.5，最大脲酶活性为 11 mmol·L·min。混合煤粉和纯煤粉的最大放热速率之差仅为 4.82 kW/m，不会影响煤产品的价值。非原位巴氏芽孢杆菌可以在煤粉微环境中正常生长代谢。最后，通过碳酸钙形成的微生物菌液矿化反应方程描述了煤粉抑制的机制，这对微生物防尘剂的进一步应用和发展具有重要意义。
