Department of Orthopaedic Surgery, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu 32200, China.
Department of Orthopaedic Surgery, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu 32200, China..
Genomics. 2023 Jul;115(4):110645. doi: 10.1016/j.ygeno.2023.110645. Epub 2023 May 23.
The processes driving ferroptosis and rotator cuff (RC) inflammation are yet unknown. The mechanism of ferroptosis and inflammation involved in the development of RC tears was investigated. The Gene Expression Omnibus database was used to obtain the microarray data relevant to the RC tears for further investigation. In this study, we created an RC tears rat model for in vivo experimental validation. For the additional function enrichment analysis, 10 hub ferroptosis-related genes were chosen to construct the correlation regulation network. In RC tears, it was discovered that genes related to hub ferroptosis and hub inflammatory response were strongly correlated. The outcomes of in vivo tests showed that RC tears were related to Cd68-Cxcl13, Acsl4-Sat1, Acsl3-Eno3, Acsl3-Ccr7, and Ccr7-Eno3 pairings in regulating ferroptosis and inflammatory response. Thus, our results show an association between ferroptosis and inflammation, providing a new avenue to explore the clinical treatment of RC tears.
导致铁死亡和肩袖（RC）炎症的过程尚不清楚。本研究旨在探讨铁死亡和炎症在 RC 撕裂发展中的机制。我们使用基因表达综合数据库（Gene Expression Omnibus database）获取与 RC 撕裂相关的微阵列数据，进行进一步研究。本研究还建立了 RC 撕裂大鼠模型进行体内实验验证。此外，选择了 10 个与铁死亡相关的关键基因，构建了相关调控网络，进行了功能富集分析。在 RC 撕裂中，发现与关键铁死亡和关键炎症反应相关的基因具有很强的相关性。体内实验结果表明，RC 撕裂与 Cd68-Cxcl13、Acsl4-Sat1、Acsl3-Eno3、Acsl3-Ccr7 和 Ccr7-Eno3 对铁死亡和炎症反应的调节有关。综上所述，我们的研究结果表明铁死亡和炎症之间存在关联，为探索 RC 撕裂的临床治疗提供了新途径。
