Jacob Ricardo Garcia Mureb, Ervolino da Silva Ana Cláudia, Chaushu Liat, Lang Niklaus Peter, Borges Duailibe de Deus Ciro, Botticelli Daniele, Rangel Garcia Júnior Idelmo
Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio 1193, Araçatuba 16015-050, SP, Brazil.
Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio 1193, Araçatuba 16015-050, SP, Brazil.
Dent J (Basel). 2023 May 4;11(5):121. doi: 10.3390/dj11050121.
This study aimed to evaluate peri-implant bone formation in rabbits after sinus grafting mediated by hydroxyapatite and beta-tricalcium phosphate (HA + β-TCP) in granule or paste configurations, concomitant with immediate implant installation.
MATERIAL & METHODS: Thirty-four rabbit maxillary sinuses were grafted with HA + β-TCP, half of which were applied in a granule and half in a paste composition. Implant placement was performed simultaneously. At 7 and 40 days postoperatively, the animals were euthanized, and samples were prepared for tomographic, microtomographic, histological, histometric (hematoxylin and eosin staining, HE), and immunohistochemical (labeling of transcription factor Runx-2 [RUNX2], vascular endothelial growth factor [VEGF], osteocalcin [OCN], and tartrate-resistant acid phosphatase [TRAP]) analysis. Implant removal torque was also measured.
On tomography, maintenance of sinus membrane integrity was observed in both the groups. Higher values of morphometric parameters evaluated by micro-CT were found in the "paste group" after seven days. At 40 days, there were no significant differences between the groups in most of the microtomographic parameters evaluated. In histological sections stained with HE, a higher percentage of newly formed bone was observed in the "granule group" after 40 days. Similar positive immunolabeling was observed for both RUNX2 and OCN in both the experimental groups. TRAP immunolabeling was similar in both groups as well. VEGF labeling increased in the "granule group", indicating a higher osteoconductive potential in this biomaterial. Similar removal torque values were observed in both groups. Thus, the two HA + β-TCP configurations showed similar healing patterns of simultaneously installed implants adjacent to sinus floor elevation. However, significantly higher bone values were observed for the "granule configuration".
The HA + β-TCP granules and paste presentations showed favorable long-term healing results, with bone formation in similar quantities and quality adjacent to the implants.
本研究旨在评估在兔上颌窦植骨后，羟基磷灰石和β-磷酸三钙（HA +β-TCP）颗粒或糊剂形态介导下，同期即刻种植时种植体周围的骨形成情况。
34只兔的上颌窦用HA +β-TCP进行植骨，其中一半使用颗粒状，一半使用糊剂。同期进行种植体植入。术后7天和40天，对动物实施安乐死，并制备样本用于断层扫描、显微断层扫描、组织学、组织计量学（苏木精和伊红染色，HE）以及免疫组织化学（转录因子Runx-2 [RUNX2]、血管内皮生长因子[VEGF]、骨钙素[OCN]和抗酒石酸酸性磷酸酶[TRAP]标记）分析。同时测量种植体的去除扭矩。
在断层扫描中，两组均观察到窦膜完整性得以维持。7天后，通过显微CT评估的形态计量学参数在“糊剂组”中值更高。40天时，在评估的大多数显微断层扫描参数方面，两组之间无显著差异。在HE染色的组织切片中，40天后“颗粒组”中观察到新形成骨的百分比更高。在两个实验组中，RUNX2和OCN均观察到相似的阳性免疫标记。两组中TRAP免疫标记也相似。“颗粒组”中VEGF标记增加，表明该生物材料具有更高的骨传导潜力。两组观察到相似的去除扭矩值。因此，两种HA +β-TCP形态显示出同期安装于上颌窦底提升附近的种植体具有相似的愈合模式。然而，“颗粒形态”的骨值显著更高。
HA +β-TCP颗粒和糊剂表现出良好的长期愈合效果，种植体周围骨形成的数量和质量相似。
