Verhasselt Hedda Luise, Ramakrishnan Elakiya, Schlag Melina, Marchesi Julian R, Buer Jan, Kleinschnitz Christoph, Hagenacker Tim, Totzeck Andreas
Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, D-45122 Essen, Germany.
Department of Neurology and Center for Translational Neuro- and Behavioral Sciences (C-TNBS), University Hospital Essen, University of Duisburg-Essen, D-45122 Essen, Germany.
J Fungi (Basel). 2023 May 13;9(5):569. doi: 10.3390/jof9050569.
An altered gut microbiota is a possible contributing pathogenic factor in myasthenia gravis (MG), an autoimmune neuromuscular disease. However, the significance of the fungal microbiome is an understudied and neglected part of the intestinal microbiome in MG. We performed a sub-analysis of the MYBIOM study including faecal samples from patients with MG ( = 41), non-inflammatory neurological disorder (NIND, = 18), chronic inflammatory demyelinating polyradiculoneuropathy (CIDP, = 6) and healthy volunteers (n = 12) by sequencing the internal transcribed spacer 2 (ITS2). Fungal reads were obtained in 51 out of 77 samples. No differences were found in alpha-diversity indices computed between the MG, NIND, CIDP and HV groups, indicating an unaltered fungal diversity and structure. Overall, four mould species ( and ) and five yeast species ( and ) were identified. Besides one MG patient with abundant , no prominent dysbiosis in the MG group of the mycobiome was found. Not all fungal sequences within all groups were successfully assigned, so further sub-analysis was withdrawn, limiting robust conclusions.
肠道微生物群改变可能是重症肌无力（MG）这一自身免疫性神经肌肉疾病的致病因素之一。然而，真菌微生物群的重要性在MG肠道微生物群中是一个研究不足且被忽视的部分。我们对MYBIOM研究进行了一项子分析，通过对内部转录间隔区2（ITS2）进行测序，纳入了MG患者（n = 41）、非炎性神经系统疾病（NIND，n = 18）、慢性炎性脱髓鞘性多发性神经根神经病（CIDP，n = 6）和健康志愿者（n = 12）的粪便样本。77个样本中有51个获得了真菌读数。在MG、NIND、CIDP和健康志愿者组之间计算的α多样性指数未发现差异，表明真菌多样性和结构未改变。总体而言，鉴定出了四种霉菌（和）和五种酵母（和）。除了一名MG患者的含量丰富外，在MG组的真菌微生物群中未发现明显的生态失调。并非所有组内的真菌序列都成功分类，因此撤回了进一步的子分析，这限制了得出有力结论。
