丁香罗勒、毛罗勒和疏花罗勒提取物的抗龋特性及其对人口腔角质形成细胞的影响。
Anti-cariogenic property of , , linn extracts and their effect on human oral keratinocytes.
作者信息
Govindaram Divyalakshmi, Kumar A Ramesh, Krishnan Rajkumar, Savithri N
机构信息
Department of Oral and Maxillofacial Pathology and Microbiology, SRM Dental College, Chennai, Tamil Nadu, India.
Department of Microbiology, SRM Dental College, Chennai, Tamil Nadu, India.
出版信息
J Oral Maxillofac Pathol. 2023 Jan-Mar;27(1):26-32. doi: 10.4103/jomfp.jomfp_151_21. Epub 2023 Mar 21.
OBJECTIVES
Dental caries is a dynamic and composite process. The multifactorial etio-pathogenesis thus influences the initiation and the progression of the disease. The prime pathogenic bacterium includes sp . The purpose of this study was to analyze the antimicrobial property of the test herbal extracts and also their effects on the human oral keratinocytes.
MATERIALS AND METHODS
The bacterial strains (American Type Culture Collection [ATCC]-25175); (ATCC 4356) and (ATCC 15987) were cultured in the specific culture media-Mitis Salivarius Bacitracin, Man Rogosa Sharpe and Enrichment media, respectively. The test extracts were exposed to the cultured plates and the mean zone of inhibition was measured. The test herbal extracts were also tested for deleterious effects on oral keratinocytes via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Independent Student's -test and analysis of variances were performed.-25175); Lactobacillus species (ATCC 4356) and A. viscosus (ATCC 15987) were cultured in the specific culture media-Mitis Salivarius Bacitracin, Man Rogosa Sharpe and Enrichment media, respectively. The test extracts were exposed to the cultured plates and the mean zone of inhibition was measured. The test herbal extracts were also tested for deleterious effects on oral keratinocytes via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Independent Student's -test and analysis of variances were performed.
RESULTS
The extracts of , and linn inhibited the growth of bacteria and the antimicrobial effect was found to be statistically significant at the neat/standard concentration (100 μg/ml). The three extracts showed a cell viability range 96%-99% indicating that the test extracts did not produce or display any deleterious effects on the oral keratinocytes.
CONCLUSIONS
The three test herbal extracts possess effective anti-cariogenic properties with near par with the efficacy of chlorhexidine and proved to be the most potent. The extracts at different concentrations also proved to be safe, noncytotoxic producing a range of 96%-99% of cell viability of the oral keratinocytes.
目的
龋齿是一个动态的复合过程。多因素病因发病机制会影响该疾病的发生和发展。主要致病细菌包括……。本研究的目的是分析受试草药提取物的抗菌特性及其对人口腔角质形成细胞的影响。
材料与方法
细菌菌株（美国典型培养物保藏中心[ATCC]-25175）；嗜酸乳杆菌（ATCC 4356）和粘性放线菌（ATCC 15987）分别在特定培养基——唾液链球菌杆菌肽培养基、曼-罗格斯-夏普培养基和富集培养基中培养。将受试提取物置于培养平板上，测量平均抑菌圈。还通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法检测受试草药提取物对口腔角质形成细胞的有害影响。进行独立样本t检验和方差分析。-25175）；嗜酸乳杆菌（ATCC 4356）和粘性放线菌（ATCC 15987）分别在特定培养基——唾液链球菌杆菌肽培养基、曼-罗格斯-夏普培养基和富集培养基中培养。将受试提取物置于培养平板上，测量平均抑菌圈。还通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法检测受试草药提取物对口腔角质形成细胞的有害影响。进行独立样本t检验和方差分析。
结果
……、……和……林奈提取物抑制了细菌生长，在纯品/标准浓度（100μg/ml）下，抗菌效果具有统计学意义。这三种提取物的细胞活力范围为96%-99%，表明受试提取物对口腔角质形成细胞未产生或显示任何有害影响。
结论
这三种受试草药提取物具有有效的抗龋特性，效果接近洗必泰，且……被证明是最有效的。不同浓度的提取物也被证明是安全的，无细胞毒性，口腔角质形成细胞的细胞活力范围为96%-99%。
Zotero 插件