Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.
J Appl Microbiol. 2010 Dec;109(6):2151-9. doi: 10.1111/j.1365-2672.2010.04847.x. Epub 2010 Sep 16.
The objective of this study was to isolate and characterize the active compound from Trachyspermum ammi seeds, exhibiting antibiofilm activity against Streptococcus mutans, a major causal organism of dental caries.
Purification of the active compound from the seeds was performed by silica gel chromatography, and spectroscopic methods (FTIR, NMR and MS) were employed for its identification and structure determination. Antibiofilm and antiadherence activities of the active compound against S. mutans were analysed. Confocal microscopy was performed to visualize the effect of the compound on biofilm structure of S. mutans. Around 50% reduction was observed in adherence at 39·06 μg ml(-1) and in biofilm at 78·13 μg ml(-1) . It was found effective against adherent cells of S. mutans, reduced water-insoluble glucan synthesis and inhibited the reduction in pH. Confocal microscopy revealed scattered cells at sub-MIC concentration of the compound, resulting in distorted biofilm architecture in contrast to clustered cells seen in control.
This study revealed a novel compound, a naphthalene derivative, isolated first time from T. ammi seeds with antibiofilm activity against S. mutans.
Trachyspermum ammi represents an interesting source of a novel compound, (4aS, 5R, 8aS) 5, 8a-di-1-propyl-octahydronaphthalen-1-(2H)-one, with a great potential to be used as a therapeutic agent against dental caries.
本研究旨在从胡芦巴种子中分离并鉴定具有抗变形链球菌生物膜活性的活性化合物，变形链球菌是龋齿的主要致病因子。
通过硅胶色谱法对种子中的活性化合物进行纯化，并采用傅里叶变换红外光谱（FTIR）、核磁共振（NMR）和质谱（MS）等光谱方法对其进行鉴定和结构测定。分析了活性化合物对变形链球菌的抗生物膜和抗黏附活性。采用共聚焦显微镜观察化合物对变形链球菌生物膜结构的影响。在 39.06μg/ml 时观察到黏附减少约 50%，在 78.13μg/ml 时观察到生物膜减少约 50%。该化合物对变形链球菌黏附细胞有效，减少了水不溶性葡聚糖的合成，并抑制了 pH 值的降低。共聚焦显微镜显示，在低于 MIC 的化合物浓度下，细胞呈散射状，导致生物膜结构扭曲，与对照中看到的聚集细胞形成对比。
本研究首次从胡芦巴种子中分离出一种具有抗变形链球菌生物膜活性的新型化合物，为萘衍生物。
胡芦巴是一种具有很大潜力的新型化合物（4aS, 5R, 8aS）-5, 8a-二-1-丙基-八氢萘-1-（2H）-酮的有趣来源，可作为治疗龋齿的潜在药物。
