Sunitha J, Krishna Swathy, Ananthalakshmi R, Jeeva J Sathiya, Girija As Smiline, Jeddy Nadeem
Reader, Department of Pathology and Microbiology, Thai Moogambigai Dental College, Chennai, Tamilnadu, India.
Student, Department of Oral Pathology, Thai Moogambigai Dental College, Chennai, Tamilnadu, India.
J Clin Diagn Res. 2017 Jun;11(6):KC01-KC04. doi: 10.7860/JCDR/2017/23602.10066. Epub 2017 Jun 1.
and are which are indigeneous to India. commonly called 'manathakkali Keerai' in Tamil, forms an indispensable part of South Indian diet. (keezhanelli in Tamil) is a widely used medicinal plant, the leaves of which have been used extensively in Ayurveda and native medicine to cure various liver ailments. The herbs and have been found to be effective against numerous enteropathogens in various in vitro studies.
To assess and compare the antibacterial efficacy of the crude alcoholic extract of the leaves of and against five cariogenic organisms.
Standard strains of the micro-organisms were obtained from ATCC (American Type Culture Collection) and MTCC (Microbial Type Culture Collection) which comprised of MTCC no. 890, MTCC no 2696, MTCC no. 10307, ATCC no. 10556 and ATCC no. 13419. The organisms obtained were revived and lawn cultured on Trypticase Soy Agar-Blood Agar (TSA-BA) and de Man, Rogosa and Sharpe (MRS) agar media. The antibacterial effect of the dried and powdered leaves of and was tested using agar well diffusion method. The zones of inhibition obtained after incubation were measured and tabulated. The antibacterial activity for the two herbs was compared using the Mann-Whitney test.
The antibacterial zones of inhibition obtained for the herb was in the range of 12.3-14.6 mm and ranged from 9.7-11.6 mm for the herb . When the zones of inhibition were compared for the herbs, showed significantly greater zones of inhibition compared to for the organisms , , and (p-value<0.05).
The alcoholic extract of leaves of and showed significant antibacterial activity against cariogenic organisms, with being more anti-cariogenic than .
[植物名称1]和[植物名称2]原产于印度。[植物名称1]在泰米尔语中通常被称为“manathakkali Keerai”，是南印度饮食中不可或缺的一部分。[植物名称2]（泰米尔语中的keezhanelli）是一种广泛使用的药用植物，其叶子在阿育吠陀医学和本土医学中被广泛用于治疗各种肝脏疾病。在各种体外研究中发现，[植物名称1]和[植物名称2]这两种草药对多种肠道病原体有效。
评估并比较[植物名称1]和[植物名称2]叶子的粗乙醇提取物对五种致龋菌的抗菌效果。
微生物标准菌株从美国典型培养物保藏中心（ATCC）和微生物型培养物保藏中心（MTCC）获得，包括变形链球菌MTCC编号890、远缘链球菌MTCC编号2696、嗜酸乳杆菌MTCC编号10307、血链球菌ATCC编号10556和黏性放线菌ATCC编号13419。获得的菌株复苏后在胰蛋白胨大豆琼脂 - 血琼脂（TSA - BA）和德氏、罗氏和夏普（MRS）琼脂培养基上进行平板培养。采用琼脂扩散法测试[植物名称1]和[植物名称2]干燥粉末状叶子的抗菌效果。孵育后测量并记录抑菌圈大小。使用曼 - 惠特尼检验比较两种草药的抗菌活性。
[植物名称1]的抑菌圈范围为12.3 - 14.6毫米，[植物名称2]的抑菌圈范围为9.7 - 11.6毫米。当比较两种草药对各菌株的抑菌圈时，对于变形链球菌、远缘链球菌、嗜酸乳杆菌和血链球菌，[植物名称1]的抑菌圈明显大于[植物名称2]（p值<0.05）。
[植物名称1]和[植物名称2]叶子的乙醇提取物对致龋菌显示出显著的抗菌活性，其中[植物名称1]的抗龋活性比[植物名称2]更强。
