Xu Shujuan, Ma Biao, Li Jiali, Su Wei, Xu Tianran, Zhang Mingzhou
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, China.
Hangzhou Quickgene Sci-Tech. Co., Ltd., Hangzhou 310018, China.
Toxics. 2023 Apr 29;11(5):417. doi: 10.3390/toxics11050417.
Drug abuse is becoming increasingly dangerous nowadays. Morphine (MOP), methamphetamine (MET) and ketamine (KET) are the most commonly abused drugs. The abuse of these drugs without supervision can cause serious harm to the human body and also endanger public safety. Developing a rapid and accurate method to screen drug suspects and thus control these drugs is essential to public safety. This paper presents a method for the simultaneous quantitative detection of these three drugs in hair by a europium nanoparticles-based fluorescence immunochromatographic assay (EuNPs-FIA). In our study, the test area of the nitrocellulose membrane was composed of three equally spaced detection lines and a quality control line. The test strip realized the quantitative analysis of the samples by detecting the fluorescence brightness of the europium nanoparticles captured on the test line within 15 min. For the triple test strip, the limits of detection of MOP, KET and MET were 0.219, 0.079 and 0.329 ng/mL, respectively. At the same time, it also showed strong specificity. The strip was stable and could be stored at room temperature for up to one year, and the average recovery rate was 85.98-115.92%. In addition, the EuNPs-FIA was validated by high-performance liquid chromatography (HPLC) analysis, and a satisfactory consistency was obtained. Compared to the current immunochromatographic methods used for detecting abused drugs in hair, this method not only increased the number of detection targets, but also ensured sensitivity, improving detection efficiency to a certain extent. The approach can also be used as an alternative to chromatography. It provides a rapid and accurate screening method for the detection of abused drugs in hair and has great application prospects in regard to public safety.
如今，药物滥用正变得越来越危险。吗啡（MOP）、甲基苯丙胺（MET）和氯胺酮（KET）是最常被滥用的药物。在无监管的情况下滥用这些药物会对人体造成严重伤害，还会危及公共安全。开发一种快速、准确的方法来筛查涉毒嫌疑人并进而管控这些药物对公共安全至关重要。本文提出了一种基于铕纳米颗粒的荧光免疫层析法（EuNPs - FIA）同时定量检测头发中这三种药物的方法。在我们的研究中，硝酸纤维素膜的检测区域由三条等间距的检测线和一条质控线组成。该试纸条通过在15分钟内检测检测线上捕获的铕纳米颗粒的荧光亮度实现了对样品的定量分析。对于三联检测试纸条，MOP、KET和MET的检测限分别为0.219、0.079和0.329 ng/mL。同时，它还表现出很强的特异性。该试纸条稳定，可在室温下保存长达一年，平均回收率为85.98 - 115.92%。此外，通过高效液相色谱（HPLC）分析对EuNPs - FIA进行了验证，获得了令人满意的一致性。与目前用于检测头发中滥用药物的免疫层析方法相比，该方法不仅增加了检测目标的数量，还确保了灵敏度，在一定程度上提高了检测效率。该方法也可作为色谱法的替代方法。它为头发中滥用药物的检测提供了一种快速、准确的筛查方法，在公共安全方面具有广阔的应用前景。
