Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Murcia, Spain.
PLoS One. 2023 May 26;18(5):e0286092. doi: 10.1371/journal.pone.0286092. eCollection 2023.
The effects of filtration (F) and alpha-amylase depletion (AD) were assessed in n = 34 saliva samples. Each saliva sample was split into three aliquots and treated as follows: (1) no treatment; (2) 0.45μm commercial filter; and (3) 0.45μm commercial filter and affinity depletion of alpha-amylase. Then, a panel of biochemical biomarkers consisting of amylase, lipase, alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), creatine kinase (CK), calcium, phosphorus, total protein, albumin, urea, creatinine, cholesterol, triglycerides, and uric acid was measured. Differences between the different aliquots were observed in all measured analytes. The most marked changes were found in triglycerides and lipase data for filtered samples, and in alpha-amylase, uric acid, triglycerides, creatinine, and calcium results in alpha-amylase-depleted aliquots. In conclusion, the salivary filtration and amylase depletion methods employed in this report caused significant changes in saliva composition measurements. Based on these results, it would be recommended to consider the possible effects of these treatments in salivary biomarkers when filtration or amylase depletion is performed.
本研究评估了过滤（F）和α-淀粉酶耗竭（AD）对 34 份唾液样本的影响。每个唾液样本均分成三份，并进行如下处理：（1）无处理；（2）0.45μm 商用滤器；（3）0.45μm 商用滤器和α-淀粉酶亲和耗尽。然后，用一系列生化标志物（包括淀粉酶、脂肪酶、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、γ-谷氨酰转移酶（GGT）、碱性磷酸酶（ALP）、肌酸激酶（CK）、钙、磷、总蛋白、白蛋白、尿素、肌酐、胆固醇、甘油三酯和尿酸）进行测量。在所有测量的分析物中，不同等分试样之间存在差异。在过滤样本中，甘油三酯和脂肪酶数据以及在α-淀粉酶耗竭样本中α-淀粉酶、尿酸、甘油三酯、肌酐和钙结果中发现了最明显的变化。总之，本报告中采用的唾液过滤和淀粉酶耗竭方法导致唾液成分测量发生了显著变化。基于这些结果，建议在进行过滤或淀粉酶耗竭时，考虑这些处理对唾液生物标志物的可能影响。
