通过化学发光免疫测定法检测钩端螺旋体。

The detection of leptospires by a chemiluminescent immunoassay.

作者信息

Waitkins S A, Hookey J V

出版信息

J Med Microbiol. 1986 Jun;21(4):353-6. doi: 10.1099/00222615-21-4-353.

Abstract

Rabbit serum hyperimmune to Leptospira interrogans var icterohaemorrhagiae serovar icterohaemorrhagiae, reference strain RGA was conjugated to the chemiluminescent label ABEI (6-[N-(4-aminobutyl)-N-ethyl amino]-2,3-dihydrophthalazine-1,4-dione) in the presence of the coupling agent, EDC (1-ethyl-3(3-dimethyl amino-propyl)-carbodiimide HCl). The luminescent conjugate was incubated with homologous and heterologous antigens. The results indicate that chemiluminescence may provide an accurate and rapid method of detecting leptospires in biological fluids.

摘要

用偶联剂1-乙基-3-(3-二甲氨基丙基)-碳二亚胺盐酸盐（EDC），将对出血性黄疸型钩端螺旋体黄疸出血血清型参考菌株RGA高度免疫的兔血清与化学发光标记物6-[N-(4-氨基丁基)-N-乙基氨基]-2,3-二氢酞嗪-1,4-二酮（ABEI）进行偶联。将发光偶联物与同源和异源抗原一起孵育。结果表明，化学发光可能为检测生物体液中的钩端螺旋体提供一种准确、快速的方法。