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用于检测问号钩端螺旋体哈焦血清型哈焦-波摩那基因型的DNA探针。

DNA probe for detection of the Leptospira interrogans serovar hardjo genotype hardjo-bovis.

作者信息

LeFebvre R B

机构信息

National Animal Disease Center, Ames, Iowa 50010.

出版信息

J Clin Microbiol. 1987 Nov;25(11):2236-8. doi: 10.1128/jcm.25.11.2236-2238.1987.

DOI:10.1128/jcm.25.11.2236-2238.1987
PMID:2826538
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269454/
Abstract

A DNA probe is described for the diagnostic and taxonomic identification of the North American cattle pathogen Leptospira interrogans genotype hardjo-bovis. The probe is specific for this genotype and does not hybridize to genomic DNA of any other leptospire pathogen commonly found in North America.

摘要

一种DNA探针被用于北美牛病原体问号钩端螺旋体hardjo-bovis基因型的诊断和分类鉴定。该探针对此基因型具有特异性，且不与北美常见的任何其他钩端螺旋体病原体的基因组DNA杂交。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1edd/269454/046f39890e9f/jcm00095-0217-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1edd/269454/c7b29a6f70e0/jcm00095-0217-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1edd/269454/046f39890e9f/jcm00095-0217-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1edd/269454/c7b29a6f70e0/jcm00095-0217-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1edd/269454/046f39890e9f/jcm00095-0217-b.jpg

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DNA probe for detection of the Leptospira interrogans serovar hardjo genotype hardjo-bovis.用于检测问号钩端螺旋体哈焦血清型哈焦-波摩那基因型的DNA探针。
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2
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Leptospira species categorized by arbitrarily primed polymerase chain reaction (PCR) and by mapped restriction polymorphisms in PCR-amplified rRNA genes.通过任意引物聚合酶链反应（PCR）以及PCR扩增的rRNA基因中的限制性酶切图谱多态性对钩端螺旋体进行分类。

本文引用的文献

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NUTRITION OF LEPTOSPIRA POMONA AND GROWTH OF 13 OTHER SEROTYPES: A SERUM-FREE MEDIUM EMPLOYING OLEIC ALBUMIN COMPLEX.波摩那钩端螺旋体的营养及其他13个血清型的生长：一种使用油酸白蛋白复合物的无血清培养基。
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Bovine leptospirosis: bacteriologic versus serologic diagnosis of cows at slaughter.牛钩端螺旋体病：屠宰母牛的细菌学诊断与血清学诊断
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A rapid alkaline extraction method for the isolation of plasmid DNA.一种用于分离质粒DNA的快速碱性提取方法。
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Characterization of Leptospira isolates from serovar hardjo by ribotyping, arbitrarily primed PCR, and mapped restriction site polymorphisms.通过核糖体分型、随机引物PCR和限制性酶切位点多态性图谱分析对来自哈焦血清型的钩端螺旋体分离株进行特征鉴定。
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Rapid and specific detection of pathogenic Leptospira species by amplification of ribosomal sequences.通过核糖体序列扩增快速、特异性检测致病性钩端螺旋体菌种
Mol Biotechnol. 1994 Aug;2(1):1-14. doi: 10.1007/BF02789285.
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Repetitive sequence element cloned from Leptospira interrogans serovar hardjo type hardjo-bovis provides a sensitive diagnostic probe for bovine leptospirosis.从问号钩端螺旋体哈焦型哈焦-波瓦血清型克隆的重复序列元件为牛钩端螺旋体病提供了一种灵敏的诊断探针。
J Clin Microbiol. 1988 Dec;26(12):2495-500. doi: 10.1128/jcm.26.12.2495-2500.1988.
7
Detection of leptospires in urine by polymerase chain reaction.通过聚合酶链反应检测尿液中的钩端螺旋体。
J Clin Microbiol. 1989 Oct;27(10):2258-62. doi: 10.1128/jcm.27.10.2258-2262.1989.
8
Relationship among selected Leptospira interrogans serogroups as determined by nucleic acid hybridization.
J Clin Microbiol. 1989 Dec;27(12):2724-9. doi: 10.1128/jcm.27.12.2724-2729.1989.
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Unique ribosome structure of Leptospira interrogans is composed of four rRNA components.
J Bacteriol. 1990 Jun;172(6):3478-80. doi: 10.1128/jb.172.6.3478-3480.1990.
10
Pulsed-field gel electrophoresis of NotI digests of leptospiral DNA: a new rapid method of serovar identification.钩端螺旋体DNA的NotI酶切脉冲场凝胶电泳：一种新的血清型鉴定快速方法。
J Clin Microbiol. 1992 Jul;30(7):1696-702. doi: 10.1128/jcm.30.7.1696-1702.1992.
Methods Enzymol. 1983;100:243-55. doi: 10.1016/0076-6879(83)00059-2.
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Main antigens as criterion for differentiating leptospiral serotypes.
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Appl Microbiol. 1973 Jun;25(6):976-80. doi: 10.1128/am.25.6.976-980.1973.
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10
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Am J Vet Res. 1986 Apr;47(4):959-63.