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厄瓜多尔农村社区健康携带者及后院动物体内和体外β-内酰胺酶与基因的共同携带情况

Co-Harboring of Beta-Lactamases and Genes in and from Healthy Carriers and Backyard Animals in Rural Communities in Ecuador.

作者信息

Bastidas-Caldes Carlos, Cisneros-Vásquez Emily, Zambrano Antonella, Mosquera-Maza Andrea, Calero-Cáceres William, Rey Joaquín, Yamamoto Yoshimasa, Yamamoto Mayumi, Calvopiña Manuel, de Waard Jacobus H

机构信息

One Health Research Group, Facultad de Ingeniería y Ciencias Aplicadas, Biotecnología, Universidad de las Américas, Quito 170124, Ecuador.

Ministerio de Salud Pública del Ecuador, Pastaza 170505, Ecuador.

出版信息

Antibiotics (Basel). 2023 May 5;12(5):856. doi: 10.3390/antibiotics12050856.

DOI:10.3390/antibiotics12050856
PMID:37237759
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10215259/
Abstract

Few studies have addressed drug resistance of Enterobacterales in rural communities in developing countries. This study aimed to determine the coexistence of extended-spectrum β-lactamase (ESBL) and carbapenemase genes in and strains carrying the gene in rural communities in Ecuador from healthy humans and their backyard animals. Sixty-two strains, thirty and thirty-two strains carrying the gene were selected from a previous study. PCR were performed for the presence of ESBLs and carbapenemase genes. The strains were further characterized, and the genetic relationship was studied with multi-locus sequencing typing (MLST) of seven housekeeping genes. Fifty-nine of the sixty-two isolates (95%) harbored at least on β-lactam resistance gene. The most prevalent ESBL genes were the genes (present in in 80% of the strains) and the gene (present in 84% of the strains). MSLT analysis revealed 28 different sequence types (ST); 15 for and 12 for , with most ST never described in humans and animals. The coexistence of and β-lactams resistant genes in and strains is alarming and threatens the efficacy of last-resort antibiotics. Our findings highlight backyard animals as a reservoir of β-lactams resistant genes.

摘要

很少有研究涉及发展中国家农村社区肠杆菌科细菌的耐药性。本研究旨在确定厄瓜多尔农村社区健康人群及其后院动物携带blaCTX-M基因的大肠杆菌和肺炎克雷伯菌中广谱β-内酰胺酶（ESBL）和碳青霉烯酶基因的共存情况。从先前的一项研究中选取了62株菌株，其中30株大肠杆菌和32株携带blaCTX-M基因的肺炎克雷伯菌。对ESBL和碳青霉烯酶基因的存在情况进行了PCR检测。对这些菌株进行了进一步鉴定，并用7个管家基因的多位点测序分型（MLST）研究了它们的亲缘关系。62株大肠杆菌分离株中有59株（95%）至少携带一种β-内酰胺抗性基因。最常见的ESBL基因是CTX-M基因（存在于80%的大肠杆菌菌株中）和TEM基因（存在于84% 的肺炎克雷伯菌菌株中）。MSLT分析揭示了28种不同的序列类型（ST）；大肠杆菌有15种，肺炎克雷伯菌有12种，大多数ST在人和动物中从未被描述过。大肠杆菌和肺炎克雷伯菌菌株中blaCTX-M和β-内酰胺抗性基因的共存令人担忧，并威胁到最后手段抗生素的疗效。我们的研究结果突出了后院动物作为β-内酰胺抗性基因库的作用

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53d8/10215259/5716225cb209/antibiotics-12-00856-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53d8/10215259/8ded2e94571e/antibiotics-12-00856-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53d8/10215259/842aa8096afe/antibiotics-12-00856-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53d8/10215259/638978d25dca/antibiotics-12-00856-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53d8/10215259/5716225cb209/antibiotics-12-00856-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53d8/10215259/8ded2e94571e/antibiotics-12-00856-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53d8/10215259/842aa8096afe/antibiotics-12-00856-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53d8/10215259/638978d25dca/antibiotics-12-00856-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53d8/10215259/5716225cb209/antibiotics-12-00856-g004.jpg

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