Spoladori Laís Fernanda de Almeida, Andriani Gabriella Maria, Castro Isabela Madeira de, Suzukawa Helena Tiemi, Gimenes Ana Carolina Ramos, Bartolomeu-Gonçalves Guilherme, Ishida Kelly, Nakazato Gerson, Pinge-Filho Phileno, Machado Rayanne Regina Beltrame, Nakamura Celso Vataru, Andrade Galdino, Tavares Eliandro Reis, Yamauchi Lucy Megumi, Yamada-Ogatta Sueli Fumie
Programa de Pós-Graduação em Microbiologia, Universidade Estadual de Londrina, Londrina CEP 86057-970, Brazil.
Laboratório de Biologia Molecular de Microrganismos, Universidade Estadual de Londrina, Londrina CEP 86057-970, Brazil.
Antibiotics (Basel). 2023 May 6;12(5):861. doi: 10.3390/antibiotics12050861.
has been found to be a persistent colonizer of human skin and a successful pathogen capable of causing potentially fatal infection, especially in immunocompromised individuals. This fungal species is usually resistant to most antifungal agents and has the ability to form biofilms on different surfaces, representing a significant therapeutic challenge. Herein, the effect of metabolites of LV strain, alone and combined with biologically synthesized silver nanoparticles (bioAgNP), was evaluated in planktonic and sessile (biofilm) cells of . First, the minimal inhibitory and fungicidal concentration values of 3.12 and 6.25 μg/mL, respectively, were determined for F4a, a semi-purified bacterial fraction. Fluopsin C and indolin-3-one seem to be the active components of F4a. Like the semi-purified fraction, they showed a time- and dose-dependent fungicidal activity. F4a and bioAgNP caused severe changes in the morphology and ultrastructure of fungal cells. F4a and indolin-3-one combined with bioAgNP exhibited synergistic fungicidal activity against planktonic cells. F4a, alone or combined with bioAgNP, also caused a significant decrease in the number of viable cells within the biofilms. No cytotoxicity to mammalian cells was detected for bacterial metabolites combined with bioAgNP at synergistic concentrations that presented antifungal activity. These results indicate the potential of F4a combined with bioAgNP as a new strategy for controlling infections.
已发现其是人类皮肤的持续定植菌，也是一种能够引发潜在致命感染的成功病原体，尤其是在免疫功能低下的个体中。这种真菌通常对大多数抗真菌药物具有抗性，并且有能力在不同表面形成生物膜，这构成了重大的治疗挑战。在此，评估了LV菌株的代谢产物单独以及与生物合成银纳米颗粒（bioAgNP）联合使用时，对[具体真菌名称]的浮游细胞和固着（生物膜）细胞的影响。首先，确定了半纯化细菌组分F4a对[具体真菌名称]的最小抑菌浓度和最小杀菌浓度值分别为3.12μg/mL和6.25μg/mL。荧光素C和吲哚-3-酮似乎是F4a的活性成分。与半纯化组分一样，它们表现出时间和剂量依赖性的杀菌活性。F4a和bioAgNP导致真菌细胞的形态和超微结构发生严重变化。F4a和吲哚-3-酮与bioAgNP联合对浮游细胞表现出协同杀菌活性。F4a单独或与bioAgNP联合使用，也会使生物膜内活细胞数量显著减少。在具有抗真菌活性的协同浓度下，未检测到细菌代谢产物与bioAgNP对哺乳动物细胞的细胞毒性。这些结果表明F4a与bioAgNP联合使用作为控制[具体真菌名称]感染的新策略具有潜力。
