猪源幽门螺杆菌样阳性胃样本中甲硝唑和四环素耐药基因的分子检测
Molecular Detection of Metronidazole and Tetracycline Resistance Genes in -Like Positive Gastric Samples from Pigs.
作者信息
Cortez Nunes Francisco, Taillieu Emily, Letra Mateus Teresa, Teixeira Sílvia, Haesebrouck Freddy, Amorim Irina
机构信息
School of Medicine and Biomedical Sciences (ICBAS), University of Porto, 4050-313 Porto, Portugal.
Institute for Research and Innovation in Health (i3S), University of Porto, 4200-135 Porto, Portugal.
出版信息
Antibiotics (Basel). 2023 May 13;12(5):906. doi: 10.3390/antibiotics12050906.
Abstract
Antimicrobial resistance is a major public health concern. The aim of this study was to assess the presence of antibiotic resistance genes, previously reported in in gastric samples of 36 pigs, in which DNA of -like organisms had been detected. Based on PCR and sequencing analysis, two samples were positive for the mutation gene, conferring tetracycline resistance, and one sample was positive for the gene with a single nucleotide polymorphism, conferring metronidazole resistance. All three amplicons showed the highest homology with -associated antibiotic resistance gene sequences. These findings indicate that acquired antimicrobial resistance may occur in -like organisms associated with pigs.
摘要
抗菌药物耐药性是一个主要的公共卫生问题。本研究的目的是评估先前在36头猪的胃样本中报道的抗生素耐药基因的存在情况，这些样本中已检测到类幽门螺杆菌的DNA。基于聚合酶链反应（PCR）和测序分析，两个样本的突变基因呈阳性，该基因赋予四环素耐药性，一个样本的基因呈阳性，带有一个单核苷酸多态性，赋予甲硝唑耐药性。所有三个扩增子与幽门螺杆菌相关的抗生素耐药基因序列具有最高的同源性。这些发现表明，获得性抗菌药物耐药性可能发生在与猪相关的类幽门螺杆菌中。
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