Molecular Detection of Metronidazole and Tetracycline Resistance Genes in -Like Positive Gastric Samples from Pigs.

Antimicrobial resistance is a major public health concern. The aim of this study was to assess the presence of antibiotic resistance genes, previously reported in in gastric samples of 36 pigs, in which DNA of -like organisms had been detected. Based on PCR and sequencing analysis, two samples were positive for the mutation gene, conferring tetracycline resistance, and one sample was positive for the gene with a single nucleotide polymorphism, conferring metronidazole resistance. All three amplicons showed the highest homology with -associated antibiotic resistance gene sequences. These findings indicate that acquired antimicrobial resistance may occur in -like organisms associated with pigs.