Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Department of General Medicine, IMSUT Hospital, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
Helicobacter. 2021 Jun;26(3):e12798. doi: 10.1111/hel.12798. Epub 2021 Apr 5.
Whereas non-Helicobacter pylori helicobacters, which are frequently detected in the stomachs of dogs and cats as a source of zoonoses, have attracted considerable attention, the role of pets in H. pylori epidemiology is unclear. In our previous study, an H. pylori infection was detected in the stomach of a dog (Dog 1). Here, we investigated the H. pylori infection status in the female offspring of Dog 1 (Dog 2) and its owner within the same household.
Biopsy specimens were obtained from the dog's owner and tested for H. pylori. DNA from gastric biopsy samples of Dog 1, gastric fluid sediment of Dog 2, and bacteria from the stomach of the owner was obtained, and Helicobacter genus- and species-specific PCRs were performed. Then, sequence analyses of the partial region of the ureAB gene were conducted.
Samples from both dogs and the owner reacted positively in the genus-specific PCR and negative in the Helicobacter felis-, Helicobacter bizzozeronii-, and Helicobacter heilmannii sensu stricto-specific PCRs. All three samples also reacted positively in the H. pylori-specific PCR. Sequences of the partial ureAB gene from all subjects were identical.
The results suggested that the two dogs and their owner were infected with an identical H. pylori strain. This report is the first to demonstrate that H. pylori can be transmitted between humans and dogs. Further studies are required to investigate the risk factors for the transmission of H. pylori between humans and dogs from the perspective of preventive epidemiology.
虽然非幽门螺杆菌螺旋杆菌常被检测到存在于狗和猫的胃中，是一种人畜共患病的来源，但宠物在幽门螺杆菌流行病学中的作用尚不清楚。在我们之前的研究中，在一只狗（狗 1）的胃中检测到了幽门螺杆菌感染。在这里，我们调查了同一家庭中狗 1 的雌性后代（狗 2）及其主人的幽门螺杆菌感染状况。
从狗的主人身上获取活检标本，并对其进行幽门螺杆菌检测。从狗 1 的胃活检样本、狗 2 的胃液沉淀物和主人胃中的细菌中提取 DNA，并进行幽门螺杆菌属和种特异性 PCR。然后，对 ureAB 基因部分区域进行序列分析。
来自狗和主人的样本在属特异性 PCR 中呈阳性，而在 Helicobacter felis、Helicobacter bizzozeronii 和 Helicobacter heilmannii sensu stricto 特异性 PCR 中呈阴性。所有三个样本在 H. pylori 特异性 PCR 中也呈阳性。来自所有研究对象的 ureAB 基因部分序列相同。
结果表明，这两只狗及其主人感染了相同的 H. pylori 菌株。本报告首次证明了 H. pylori 可以在人类和狗之间传播。需要进一步研究从预防流行病学的角度探讨 H. pylori 在人类和狗之间传播的危险因素。
