基于 PCR 的人类和动物中幽门螺杆菌和非幽门螺杆菌物种的检测，这些动物具有人畜共患病感染的潜力。

PCR-based detection of Helicobacter pylori and non-Helicobacter pylori species among humans and animals with potential for zoonotic infections.

机构信息

Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Suez Canal University, 41522, 4.5 Km Ring Road, Ismailia, Egypt.

Microbiology and Immunology Department, Faculty of Veterinary Medicine, Suez Canal University, Egypt.

出版信息

Pol J Vet Sci. 2021 Sep;24(3):445-450. doi: 10.24425/pjvs.2021.138737.

DOI:10.24425/pjvs.2021.138737

PMID:34730306

Abstract

Helicobacter species have been reported in animals, some of which are of zoonotic importance. This study aimed to detect Helicobacter species among human and animal samples using conventional PCR assays and to identify their zoonotic potentials. Helicobacter species was identified in human and animal samples by genus-specific PCR assays and phylogenetic analysis of partial sequencing of the 16S ribosomal RNA gene. The results revealed that Helicobacter species DNA was detected in 13 of 29 (44.83%) of the human samples. H. pylori was identified in 2 (15.38%), and H. bovis was detected in 4 (30.77%), whereas 7 (53.85%) were unidentified. H. bovis and H. heilmannii were prevalent among the animal samples. Phylogenetic analysis revealed bootstrapping of sequences with H. cinaedi in camel, H. rappini in sheep and humans, and Wollinella succinogenes in humans. In conclusion, the occurrence of non-H. pylori infections among human and animal samples suggested zoonotic potentials.

摘要

已在动物中报告了螺旋杆菌属物种，其中一些具有动物源重要性。本研究旨在使用常规 PCR 检测人类和动物样本中的螺旋杆菌属物种，并确定其动物源潜力。通过属特异性 PCR 检测和 16S 核糖体 RNA 基因部分测序的系统发育分析，鉴定了人类和动物样本中的螺旋杆菌属物种。结果显示，在 29 个人类样本中的 13 个样本（44.83%）中检测到螺旋杆菌属物种 DNA。鉴定出 2 个（15.38%）为 H. pylori，4 个（30.77%）为 H. bovis，而 7 个（53.85%）无法鉴定。牛种螺旋杆菌和 H. heilmannii 在动物样本中较为流行。系统发育分析显示，骆驼中的 H. cinaedi、绵羊和人类中的 H. rappini 以及人类中的 Wollinella succinogenes 序列的自举值较高。综上所述，人类和动物样本中存在非 H. pylori 感染表明存在动物源潜力。