Shang Yanjie, Yang Fengqin, Ngando Fernand Jocelin, Zhang Xiangyan, Feng Yakai, Ren Lipin, Guo Yadong
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China.
Department of Forensic Medicine, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi 830017, China.
Animals (Basel). 2023 May 11;13(10):1607. doi: 10.3390/ani13101607.
(Robineau-Desvoidy, 1830) has the potential to estimate the minimum postmortem interval (PMI). Development data and intra-puparial age estimation are significant for PMI estimation. Previous research has focused on constant temperatures, although fluctuating temperatures are a more real scenario at a crime scene. The current study examined the growth patterns of under constant (25.75 °C) and fluctuating temperatures (18-36 °C; 22-30 °C). Furthermore, differentially expressed genes, attenuated total reflectance Fourier-transform infrared spectroscopy, and cuticular hydrocarbons of during the intra-puparial period were used to estimate age. The results indicated that at fluctuating temperatures took longer to develop and had a lower pupariation rate, eclosion rate, and pupal weight than the group at constant temperatures did. Moreover, we found that six DEG expression profiles and ATR-FTIR technology, CHCs detection methods, and chemometrics can potentially estimate the intra-puparial age of at both constant and fluctuating temperatures. The findings of the study support the use of for PMI estimation and encourage the use of entomological evidence in forensic practice.
(罗比诺 - 德沃伊迪,1830年)有潜力估计最短死后间隔时间(PMI)。发育数据和蛹内年龄估计对于PMI估计很重要。先前的研究集中在恒温条件下,尽管在犯罪现场温度波动是更实际的情况。当前研究考察了在恒温(25.75℃)和温度波动(18 - 36℃;22 - 30℃)条件下[此处原文缺失研究对象,推测是某种昆虫]的生长模式。此外,利用蛹内期[此处原文缺失研究对象,推测是某种昆虫]的差异表达基因、衰减全反射傅里叶变换红外光谱以及表皮碳氢化合物来估计年龄。结果表明,与恒温组相比,温度波动条件下[此处原文缺失研究对象,推测是某种昆虫]发育所需时间更长,化蛹率、羽化率和蛹重更低。此外,我们发现六种差异表达基因表达谱以及衰减全反射傅里叶变换红外光谱技术、表皮碳氢化合物检测方法和化学计量学能够在恒温和温度波动条件下潜在地估计[此处原文缺失研究对象,推测是某种昆虫]的蛹内年龄。该研究结果支持将[此处原文缺失研究对象,推测是某种昆虫]用于PMI估计,并鼓励在法医实践中使用昆虫学证据。