Ngando Fernand Jocelin, Zhang Xiangyan, Qu Hongke, Zhang Changquan, Yang Fengqin, Feng Yakai, Shang Yanjie, Chen Sile, Ren Lipin, Guo Yadong
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China.
Cancer Research Institute, School of Basic Medical Sciences, Central South University, Changsha 410013, China.
Animals (Basel). 2023 Jul 25;13(15):2402. doi: 10.3390/ani13152402.
Flesh flies (Diptera: Sarcophagidae) are regarded as significant in medical and veterinary entomology, and their development models can be utilized as considerable markers to ascertain the minimum postmortem interval (PMImin). In this research, we explored the growth cycle and larval body length of Macquart 1839 (Diptera: Sarcophagidae) reared under variable temperatures ranging from 15.7 to 31.1 °C, with an average of 24.55 °C and relative humidity ranges from 31.4 to 82.8% and at six fixed temperatures of 15, 20, 25, 30, 32, and then 35 °C. Moreover, pteridine from the head was used to assess adult age grading. Our results allowed us to provide three development models: the isomorphen chart, the isomegalen chart, and the thermal summation models. The time taken for to complete its development from larviposition to adult emergence at constant temperatures of 15, 20, 25, 30, 32, and 35 °C was 1256.3 ± 124.2, 698.6 ± 15.1, 481.8 ± 35.7, 366.0 ± 13.5, and 295.8 ± 20.5 h, respectively, except 35 °C, where all pupae were unable to attain adulthood. They lasted 485.8 ± 5.4 h under variable temperatures. The minimum developmental limit () temperature and the thermal summation constant () of were 9.31 ± 0.55 °C and 7290.0 ± 388.4 degree hours, respectively. The increase in pteridine content exhibited variations across different temperatures. There was quite a considerable distinction in the pteridine contents of male and female at 15 °C ( = 0.0075) and 25 °C ( = 0.0213). At 32 °C and variable temperatures, the pteridine content between female and male was not statistically divergent. However, temperature and gender remain the main factors influencing the pteridine content in the head of We aim to provide detailed developmental data on that can be used as a valuable resource for future research and PMI estimation.
麻蝇（双翅目：麻蝇科）在医学和兽医昆虫学领域被视为重要研究对象，其发育模型可作为确定最短死后间隔时间（PMImin）的重要指标。在本研究中，我们探究了巴氏亚麻蝇Macquart 1839（双翅目：麻蝇科）在15.7至31.1 °C（平均24.55 °C）的可变温度以及31.4至82.8%的相对湿度条件下，和在15、20、25、30、32及35 °C这六个固定温度下的生长周期和幼虫体长。此外，还利用头部的蝶啶来评估成虫的年龄分级。我们的研究结果得出了三种发育模型：同形图、同大图和热积温模型。在15、20、25、30、32和35 °C恒温条件下，巴氏亚麻蝇从产幼虫到成虫羽化所需的时间分别为1256.3 ± 124.2、698.6 ± 15.1、481.8 ± 35.7、366.0 ± 13.5和295.8 ± 20.5小时，除了在35 °C时所有蛹均无法发育至成虫阶段。在可变温度条件下，其发育历时485.8 ± 5.4小时。巴氏亚麻蝇的最低发育极限温度和热积温常数分别为9.31 ± 0.55 °C和7290.0 ± 388.4度日。蝶啶含量在不同温度下呈现出变化。在15 °C（P = 0.0075）和25 °C（P = 0.0213）时，巴氏亚麻蝇雌雄个体的蝶啶含量存在显著差异。在32 °C和可变温度条件下，巴氏亚麻蝇雌雄个体之间的蝶啶含量无统计学差异。然而，温度和性别仍是影响巴氏亚麻蝇头部蝶啶含量的主要因素。我们旨在提供关于巴氏亚麻蝇详细的发育数据，这些数据可作为未来研究和PMI估计的宝贵资源。
