National Institute of Science and Technology on Plant Physiology under Stress Conditions, Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa 36570-900, MG, Brazil.
Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany.
Biomolecules. 2023 Apr 24;13(5):731. doi: 10.3390/biom13050731.
Among the adenylate carriers identified in , only the AMP/ATP transporter shows increased expression in roots under waterlogging stress conditions. Here, we investigated the impact of a reduced expression of in plants submitted to waterlogging conditions. For this purpose, an T-DNA mutant and two antisense lines were evaluated. Following waterlogging, deficiency resulted in a reduced maximum quantum yield of PSII electron transport (significantly for and antisense Line 10), indicating a higher impact caused by the stress in the mutants. In addition, deficient lines showed higher levels of AMP in roots under nonstress condition. This result indicates that the downregulation of impacts the levels of adenylates. -deficient plants exhibited a differential expression pattern of hypoxia-related genes with an increase in non-fermenting-related-kinase 1 () expression and upregulation of adenylate kinase () under stress and non-stress conditions. Together, these results indicated that the lower expression of is associated with an early "hypoxic status" due to the perturbation of the adenylate pool caused by reduced AMP import by mitochondria. This perturbation, which is sensed by SnRK1, results in a metabolic reprogramming associated with early induction of the fermentative pathway in deficient plants.
在鉴定的腺苷酸载体中，只有 AMP/ATP 转运蛋白在淹水胁迫条件下在根中表现出表达增加。在这里，我们研究了在淹水条件下提交的 植物中 表达降低的影响。为此，评估了一个 T-DNA 突变体和两个 反义系。在水淹后， 缺乏导致 PSII 电子传递的最大量子产量降低（对 和反义系 10 线显著），表明突变体受到的胁迫影响更大。此外，缺乏 的系在非胁迫条件下根中 AMP 水平更高。这一结果表明， 的下调会影响腺苷酸的水平。 -缺乏植物表现出与缺氧相关基因的差异表达模式，在胁迫和非胁迫条件下，非发酵相关激酶 1（）表达增加，腺苷酸激酶（）上调。总之，这些结果表明，由于线粒体减少 AMP 摄取导致腺苷酸池的扰动， 的低表达与早期“缺氧状态”相关。这种扰动被 SnRK1 感知，导致与发酵途径早期诱导相关的代谢重编程在 缺乏植物中。
