Department of Chemistry, Biology, and Biotechnology, University of Perugia, 06123 Perugia, Italy.
Institute of Bioscience and Bioresources (IBBR), National Research Council of Italy (CNR), 06128 Perugia, Italy.
Int J Mol Sci. 2023 May 10;24(10):8554. doi: 10.3390/ijms24108554.
Exposure to high light intensity (HL) and cold treatment (CT) induces reddish pigmentation in , an aquatic fern. Nevertheless, how these conditions, alone or in combination, influence growth and pigment synthesis remains to be fully elucidated. Likewise, the regulatory network underpinning the accumulation of flavonoids in ferns is still unclear. Here, we grew under HL and/or CT conditions for 20 days and evaluated the biomass doubling time, relative growth rate, photosynthetic and non-photosynthetic pigment contents, and photosynthetic efficiency by chlorophyll fluorescence measurements. Furthermore, from the genome, we mined the homologs of , , and genes, which form the MBW flavonoid regulatory complex in higher plants, to investigate their expression by qRT-PCR. We report that optimizes photosynthesis at lower light intensities, regardless of the temperature. In addition, we show that CT does not severely hamper growth, although it causes the onset of photoinhibition. Coupling CT with HL stimulates the accumulation of flavonoids, which likely prevents irreversible photoinhibition-induced damage. Although our data do not support the formation of MBW complexes, we identified candidate and regulators of flavonoids. Overall, the present findings are of fundamental and pragmatic relevance to 's biology.
强光（HL）和冷处理（CT）暴露会导致水生蕨类植物 的红色素沉着。然而，这些条件（单独或组合）如何影响 的生长和色素合成仍有待充分阐明。同样，蕨类植物中黄酮类化合物积累的调控网络仍不清楚。在这里，我们将 在 HL 和/或 CT 条件下培养 20 天，并通过叶绿素荧光测量评估生物量倍增时间、相对生长率、光合和非光合色素含量以及光合作用效率。此外，我们从 基因组中挖掘出与高等植物 MBW 黄酮类调控复合物形成的 、 和 基因的同源物，通过 qRT-PCR 研究它们的表达。我们报告说，无论温度如何， 在较低的光强度下优化光合作用。此外，我们表明 CT 不会严重阻碍 的生长，尽管它会导致光抑制的发生。将 CT 与 HL 结合使用会刺激黄酮类化合物的积累，这可能防止不可逆的光抑制诱导损伤。尽管我们的数据不支持 MBW 复合物的形成，但我们确定了黄酮类化合物的候选 和 调节剂。总的来说，这些发现对 的生物学具有基础和实际意义。
