Dolati Masoumeh, Tafvizi Farzaneh, Salehipour Masoud, Movahed Tahereh Komeili, Jafari Parvaneh
Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran.
Cellular and Molecular Resarch Center, Qom University of Medical Sciences, Qom, Iran.
Iran J Microbiol. 2021 Dec;13(6):839-847. doi: 10.18502/ijm.v13i6.8089.
Secondary metabolites in the supernatants of probiotic microorganisms have shown anticancer effects. The present study was aimed to investigate the cytotoxicity of supernatants and their role in apoptosis induction in MCF7 cancer cells.
The inhibition of MCF7 cancer cells by supernatants was assessed by MTT assay at three exposure times of 24, 48, and 72 h. Apoptosis induction was explored by flow cytometry while the expression levels of , , , and were examined by real-time PCR and compared with normal HFF cells.
supernatants exhibited inhibitory effects on MCF7 cells in a concentration-dependent and time-dependent manner; while lower cytotoxic effects were observed in normal HFF cells. The increase in the expression of , and genes and the decrease in the anti-apoptotic gene of , along with the flow cytometry results, confirmed the induction of apoptosis in the cancer cells.
Regarding the cytotoxic influence of supernatants against breast cancer cells, this bacterium can be considered as a potential candidate for a novel therapeutic strategy with lower side effects which of course requires further investigations.
益生菌微生物上清液中的次生代谢产物已显示出抗癌作用。本研究旨在探讨上清液的细胞毒性及其在诱导MCF7癌细胞凋亡中的作用。
通过MTT法在24、48和72小时这三个暴露时间评估上清液对MCF7癌细胞的抑制作用。通过流式细胞术探究凋亡诱导情况，同时通过实时PCR检测 、 、 和 的表达水平，并与正常HFF细胞进行比较。
上清液对MCF7细胞呈现浓度依赖性和时间依赖性抑制作用；而在正常HFF细胞中观察到较低的细胞毒性作用。 、 和 基因表达增加以及抗凋亡基因 表达降低，连同流式细胞术结果，证实了癌细胞中凋亡的诱导。
鉴于上清液对乳腺癌细胞的细胞毒性影响，这种细菌可被视为一种具有较低副作用的新型治疗策略的潜在候选者，当然这还需要进一步研究。
