Department of Pathobiology, School of Veterinary Medicine, Shiraz University, 71345-1731, Shiraz, Iran.
Vet Res Commun. 2023 Jun;47(2):713-721. doi: 10.1007/s11259-022-10032-7. Epub 2022 Nov 3.
Staphylococcus pseudintermedius (S. pseudintermedius), found on dogs' skin and mucous membranes, can act as an opportunistic pathogen causing skin, ear, and other tissue infections. Due to the possibility of zoonotic transmission of them, it is necessary to investigate the prevalence of S. pseudintermedius, especially the antimicrobial-resistant strains, in pets. In this study, the prevalence, antimicrobial resistance patterns, and biofilm formation ability of methicillin-resistant S. pseudintermedius (MRSP) were investigated and compared in 50 healthy dogs and 50 dogs with skin infections. The prevalence of S. pseudintermedius was not significantly different between healthy dogs (40%) and dogs with skin infections (50%). No significant difference was found in the prevalence of MRSP between healthy dogs (12%) and dogs with skin infections (18%). A total of 81.8% of S. pseudintermedius isolates were biofilm producers. The frequencies of antibiotic resistance (except for gentamicin), multidrug resistance (MDR), and biofilm formation ability were not significantly different between S. pseudintermedius isolates of healthy dogs and dogs with skin infections. The frequencies of resistance to penicillin and tetracycline, MDR, and biofilm production abilities were significantly higher among MRSP than methicillin-susceptible S. pseudintermedius (MSSP). The frequency of oxacillin resistance was significantly higher among weak or moderate biofilm producers than non-biofilm producers. The frequency of resistance to erythromycin was significantly higher among moderate biofilm producers than non-biofilm producers or weak biofilm producers. High frequencies of biofilm-producer S. pseudintermedius isolates and their resistance to antibiotics can affect the success of treatment of infections caused by these strains.
中间葡萄球菌（Staphylococcus pseudintermedius，S. pseudintermedius）存在于狗的皮肤和黏膜上，可作为机会性病原体引起皮肤、耳朵和其他组织感染。由于它们可能会发生人畜共患传播，因此有必要调查宠物中中间葡萄球菌的流行情况，尤其是耐抗生素菌株。在这项研究中，调查并比较了 50 只健康犬和 50 只患有皮肤感染的犬中耐甲氧西林中间葡萄球菌（methicillin-resistant S. pseudintermedius，MRSP）的流行率、抗生素耐药模式和生物膜形成能力。健康犬（40%）和皮肤感染犬（50%）中中间葡萄球菌的流行率无显著差异。健康犬（12%）和皮肤感染犬（18%）中 MRSP 的流行率也无显著差异。总共 81.8%的中间葡萄球菌分离株是生物膜生产者。健康犬和皮肤感染犬的中间葡萄球菌分离株的抗生素耐药（除庆大霉素外）、多药耐药（multidrug resistance，MDR）和生物膜形成能力的频率没有显著差异。MRSP 对青霉素和四环素的耐药率、MDR 和生物膜形成能力显著高于甲氧西林敏感中间葡萄球菌（methicillin-susceptible S. pseudintermedius，MSSP）。弱或中度生物膜生产者的耐苯唑西林频率显著高于非生物膜生产者。中度生物膜生产者的红霉素耐药频率显著高于非生物膜生产者或弱生物膜生产者。高频率的生物膜生产者中间葡萄球菌分离株及其对抗生素的耐药性可能会影响这些菌株引起的感染治疗的成功。
