In Vitro Antimicrobial Activity of Leaf Extract and Some Topical Agents against Methicillin-Resistant and Methicillin-Susceptible Strains from Canine Pyoderma.

As multidrug-resistant methicillin-resistant staphylococci (MRS) is becoming more prevalent in canine pyoderma, the discovery of new therapeutic options is required. This study aimed to test the antimicrobial activity of crude leaf extract and some topical antimicrobial agents against canine clinical strains by determining the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The results showed that the mean MICs of chlorhexidine, miconazole, crude leaf extract, azelaic acid, and benzoyl peroxide against strains were 1.41, 1.62, 252.78, 963.49, and 1342.70 mg/L, respectively. Therefore, betel leaf extract demonstrated a superior efficacy to azelaic acid and benzoyl peroxide. Furthermore, the ratio of MBC/MIC of betel leaf extract was 1.75, indicating its bactericidal action. When applied to methicillin-resistant (MRSP) and methicillin-susceptible (MSSP), betel leaf extract was equally efficient towards both groups. strains were more susceptible to betel leaf extract than subsp. . In gas chromatography-mass spectrometry analysis, eugenol and hydroxychavicol appeared to be the major components of betel leaf extract. Given its efficacy, dogs with pyoderma could benefit from the use of betel leaf extract as a topical antimicrobial alternative.