Braga P C, Bossi R, Allegra L
Methods Find Exp Clin Pharmacol. 1986 May;8(5):321-6.
"Mucociliary clearance", from a clinical point of view, is one of the most important defense mechanisms of the respiratory mucosa. Clinicians, pneumologists and pharmacologists are greatly interested in methods investigating the cilia, considered the biological engine that continuously moves the physiological and/or pathological bronchial mucous secretions. This paper describes a method for the study of ciliary activity in man. By a simple brushing technique it is possible to obtain samples of ciliated respiratory epithelium, and the ciliary beat can be observed immediately by microscopy (Nomarsky differential interference contrast) in a small thermostatic perfusion chamber. The ciliary image is visualized on a TV monitor by means of a TV camera. Using a new type of digital counter with a phototransistor probe, the beat rate of a single cilium can be measured directly by the monitor screen under physiological or pathological conditions or under drug perfusion.
从临床角度来看，“黏液纤毛清除功能”是呼吸道黏膜最重要的防御机制之一。临床医生、肺病学家和药理学家对研究纤毛的方法极为感兴趣，纤毛被视为持续推动生理性和/或病理性支气管黏液分泌物的生物引擎。本文描述了一种研究人体纤毛活动的方法。通过一种简单的刷取技术，可以获取纤毛呼吸道上皮细胞样本，并且能够在一个小型恒温灌注室内通过显微镜（诺马斯基微分干涉对比法）立即观察到纤毛摆动。借助电视摄像机，纤毛图像在电视监视器上显示出来。使用一种带有光电晶体管探头的新型数字计数器，在生理或病理条件下或药物灌注时，可以直接通过监视器屏幕测量单个纤毛的摆动频率。
