Immunology Department, Beni-Messous Teaching Hospital, Faculty of Pharmacy, University of Algiers, Algiers 16000, Algeria.
Epidemiology Department, Beni-Messous Teaching Hospital, Faculty of Medicine, University of Algiers, Algiers 16000, Algeria.
Viruses. 2023 May 17;15(5):1179. doi: 10.3390/v15051179.
Multiple assays have been developed for the characterization of the functional activation of SARS-CoV-2 specific T-cells. This study was conducted to assess the post-vaccination and post-infection T cell response, as detected by the QuantiFERON-SARS-CoV-2 assay using the combination of three SARS-CoV-2 specific antigens (Ag1, Ag2 and Ag3). An amount of 75 participants with different infection and vaccination backgrounds were recruited for the evaluation of humoral and cellular immune responses. An elevated IFN-γ response in at least one Ag tube was observed in 69.2% of convalescent subjects and 63.9% of vaccinated ones. Interestingly, in a healthy unvaccinated case and three convalescents with negative IgG-RBD, we detected a positive QuantiFERON test after stimulation with Ag3. The majority of the T cell responders reacted simultaneously to the three SARS-CoV-2 specific antigens, and Ag3 demonstrated the highest rate of reactivity. At univariable analysis, the only factor that was associated with an absence of a cellular response was time from blood collection, being less than 30 days (OR:3.5, CI95% [1.15-10.50], = 0.028). Overall, the inclusion of Ag3 improved the performance of the QuantiFERON-SARS-CoV-2 and showed a particular interest among subjects who fail to achieve a measurable antibody response after infection or vaccination.
已经开发出多种测定方法来描述 SARS-CoV-2 特异性 T 细胞的功能激活。本研究旨在评估接种疫苗和感染后 T 细胞的反应，方法是使用三种 SARS-CoV-2 特异性抗原（Ag1、Ag2 和 Ag3）组合，通过 QuantiFERON-SARS-CoV-2 测定来检测。招募了 75 名具有不同感染和接种背景的参与者，评估体液和细胞免疫反应。在 69.2%的恢复期患者和 63.9%的接种疫苗者中，至少有一个 Ag 管中的 IFN-γ 反应升高。有趣的是，在一个健康的未接种疫苗的病例和三个 IgG-RBD 阴性的恢复期患者中，我们在刺激 Ag3 后检测到 QuantiFERON 测试呈阳性。大多数 T 细胞反应者同时对三种 SARS-CoV-2 特异性抗原产生反应，Ag3 的反应率最高。在单变量分析中，与细胞反应缺失相关的唯一因素是采血时间，小于 30 天（OR：3.5，95%CI [1.15-10.50]， = 0.028）。总的来说，Ag3 的加入提高了 QuantiFERON-SARS-CoV-2 的性能，并且在感染或接种疫苗后无法产生可测量的抗体反应的患者中显示出特别的兴趣。
