有机溶剂和温度对酵母3-磷酸甘油酸激酶从免疫吸附剂上解吸的影响。
The effects of organic solvents and temperature on the desorption of yeast 3-phosphoglycerate kinase from immunoadsorbent.
作者信息
Andersson K K, Benyamin Y, Douzou P, Balny C
出版信息
J Immunol Methods. 1979;25(4):375-81. doi: 10.1016/0022-1759(79)90029-2.
PMID:372447
Abstract
Physicochemical parameters governing the elution of yeast 3-phosphoglycerate kinase from its immunoadsorbent were studied. Non-denaturing elution conditions were determined (alkali medium containing 50%, v/v, ethylene glycol) and the method was applied to one-step isolation of enzyme from a crude yeast preparation.
摘要
研究了控制酵母3-磷酸甘油酸激酶从其免疫吸附剂上洗脱的物理化学参数。确定了非变性洗脱条件（含50%，v/v，乙二醇的碱性介质），并将该方法应用于从酵母粗制品中一步分离酶。
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