• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

有机溶剂和温度对酵母3-磷酸甘油酸激酶从免疫吸附剂上解吸的影响。

The effects of organic solvents and temperature on the desorption of yeast 3-phosphoglycerate kinase from immunoadsorbent.

作者信息

Andersson K K, Benyamin Y, Douzou P, Balny C

出版信息

J Immunol Methods. 1979;25(4):375-81. doi: 10.1016/0022-1759(79)90029-2.

DOI:10.1016/0022-1759(79)90029-2
PMID:372447
Abstract

Physicochemical parameters governing the elution of yeast 3-phosphoglycerate kinase from its immunoadsorbent were studied. Non-denaturing elution conditions were determined (alkali medium containing 50%, v/v, ethylene glycol) and the method was applied to one-step isolation of enzyme from a crude yeast preparation.

摘要

研究了控制酵母3-磷酸甘油酸激酶从其免疫吸附剂上洗脱的物理化学参数。确定了非变性洗脱条件（含50%，v/v，乙二醇的碱性介质），并将该方法应用于从酵母粗制品中一步分离酶。

相似文献

1
The effects of organic solvents and temperature on the desorption of yeast 3-phosphoglycerate kinase from immunoadsorbent.有机溶剂和温度对酵母3-磷酸甘油酸激酶从免疫吸附剂上解吸的影响。
J Immunol Methods. 1979;25(4):375-81. doi: 10.1016/0022-1759(79)90029-2.
2
Isolation and characterization of the yeast 3-phosphoglycerokinase gene (PGK) by an immunological screening technique.通过免疫筛选技术分离和鉴定酵母3-磷酸甘油酸激酶基因（PGK）。
J Biol Chem. 1980 Dec 25;255(24):12073-80.
3
Organic solvents and temperature effects on desorption from immunoadsorbents. DNP-BSA anti-DNP as a model.
J Immunol Methods. 1978;23(1-2):17-21. doi: 10.1016/0022-1759(78)90105-9.
4
Yeast phosphoglycerate kinase purified by affinity elution has tightly bound 3-phosphoglycerate.通过亲和洗脱纯化的酵母磷酸甘油酸激酶紧密结合3-磷酸甘油酸。
FEBS Lett. 1981 Jun 15;128(2):353-5. doi: 10.1016/0014-5793(81)80115-9.
5
Yeast phosphoglycerate kinase -- evidence from affinity elution studies for conformational changes on binding of substrates.
FEBS Lett. 1979 Oct 1;106(1):239-42. doi: 10.1016/0014-5793(79)80736-x.
6
Purification of 3-phosphoglycerate kinase from diverse sources by affinity elution chromatography.通过亲和洗脱色谱法从不同来源纯化3-磷酸甘油酸激酶。
Biochem J. 1978 Oct 1;175(1):311-9. doi: 10.1042/bj1750311.
7
3-Phosphoglycerate kinase from bovine liver and yeast.来自牛肝和酵母的3-磷酸甘油酸激酶。
Methods Enzymol. 1982;90 Pt E:115-20. doi: 10.1016/s0076-6879(82)90116-1.
8
Phosphoglycerate kinase from spinach, blue-green algae, and yeast.
Methods Enzymol. 1982;90 Pt E:110-4. doi: 10.1016/s0076-6879(82)90115-x.
9
Polyamide resin: a novel immunoadsorbent. I. Preparation of polyamide resin-antigen conjugates for use in a solid phase assay for radiolabeled antibody synthesized in vitro.聚酰胺树脂：一种新型免疫吸附剂。I. 用于体外合成放射性标记抗体的固相分析的聚酰胺树脂-抗原缀合物的制备。
Appl Biochem Biotechnol. 1983 Apr;8(2):105-14. doi: 10.1007/BF02778091.
10
Large-scale preparation of phosphoglycerate kinase from Saccharomyces cerevisiae using Cibacron Blue-Sepharose 4B pseudoaffinity chromatography.利用汽巴蓝-琼脂糖凝胶4B假亲和色谱法大规模制备酿酒酵母磷酸甘油酸激酶
Anal Biochem. 1979 Feb;93(1):46-51.

引用本文的文献

1
Purification of a Mycoplasma pneumoniae adhesin by monoclonal antibody affinity chromatography.通过单克隆抗体亲和层析法纯化肺炎支原体粘附素
J Bacteriol. 1984 Feb;157(2):678-80. doi: 10.1128/jb.157.2.678-680.1984.
2
Structural variations in actins. A study of the immunological reactivity of the N-terminal region.肌动蛋白的结构变异。N端区域免疫反应性的研究。
Biochem J. 1986 Jan 1;233(1):193-7. doi: 10.1042/bj2330193.