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通过单克隆抗体亲和层析法纯化肺炎支原体粘附素

Purification of a Mycoplasma pneumoniae adhesin by monoclonal antibody affinity chromatography.

作者信息

Leith D K, Baseman J B

出版信息

J Bacteriol. 1984 Feb;157(2):678-80. doi: 10.1128/jb.157.2.678-680.1984.

DOI:10.1128/jb.157.2.678-680.1984
PMID:6420394
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215303/
Abstract

A 165,000-dalton surface protein of Mycoplasma pneumoniae, designated protein P1, appears to be the major attachment ligand of the pathogen. We employed monoclonal antibody affinity chromatography to obtain purified protein P1.

摘要

肺炎支原体的一种165,000道尔顿的表面蛋白，命名为P1蛋白，似乎是该病原体的主要黏附配体。我们采用单克隆抗体亲和层析法来获得纯化的P1蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0955/215303/fcd28fadc584/jbacter00237-0343-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0955/215303/8c52a797cae9/jbacter00237-0342-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0955/215303/fcd28fadc584/jbacter00237-0343-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0955/215303/8c52a797cae9/jbacter00237-0342-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0955/215303/fcd28fadc584/jbacter00237-0343-a.jpg

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1
Purification of a Mycoplasma pneumoniae adhesin by monoclonal antibody affinity chromatography.通过单克隆抗体亲和层析法纯化肺炎支原体粘附素
J Bacteriol. 1984 Feb;157(2):678-80. doi: 10.1128/jb.157.2.678-680.1984.
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引用本文的文献

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Appl Environ Microbiol. 1985 Aug;50(2):431-7. doi: 10.1128/aem.50.2.431-437.1985.
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Detection of the major adhesin P1 in triton shells of virulent Mycoplasma pneumoniae.在强毒肺炎支原体的曲通外壳中检测主要黏附素P1
Infect Immun. 1985 Dec;50(3):944-6. doi: 10.1128/iai.50.3.944-946.1985.
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Detection of Mycoplasma pneumoniae adhesin (P1) in the nonhemadsorbing population of virulent Mycoplasma pneumoniae.

本文引用的文献

1
Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody.肺炎支原体黏附素通过单克隆抗体定位于顶端结构。
Nature. 1982 Aug 19;298(5876):765-7. doi: 10.1038/298765a0.
2
Molecular basis for cytadsorption of Mycoplasma pneumoniae.肺炎支原体细胞吸附的分子基础。
J Bacteriol. 1982 Sep;151(3):1514-22. doi: 10.1128/jb.151.3.1514-1522.1982.
3
Mycoplasma pneumoniae proteins that selectively bind to host cells.选择性结合宿主细胞的肺炎支原体蛋白。
在强毒力肺炎支原体的非血细胞吸附群体中检测肺炎支原体黏附素（P1）
Infect Immun. 1985 Aug;49(2):457-8. doi: 10.1128/iai.49.2.457-458.1985.
4
Adherence inhibition assay: a specific serological test for detection of antibodies to Mycoplasma pneumoniae.黏附抑制试验：一种用于检测肺炎支原体抗体的特异性血清学检测方法。
Eur J Clin Microbiol. 1985 Apr;4(2):113-8. doi: 10.1007/BF02013574.
5
Cloning and sequence analysis of cytadhesin P1 gene from Mycoplasma pneumoniae.肺炎支原体细胞黏附素P1基因的克隆与序列分析
Infect Immun. 1987 Dec;55(12):3023-9. doi: 10.1128/iai.55.12.3023-3029.1987.
6
A 168-kilodalton protein of Mycoplasma pneumoniae used as antigen in a dot enzyme-linked immunosorbent assay.肺炎支原体的一种168千道尔顿蛋白，用作斑点酶联免疫吸附测定中的抗原。
Eur J Clin Microbiol. 1986 Aug;5(4):435-40. doi: 10.1007/BF02075700.
Infect Immun. 1982 Jul;37(1):382-6. doi: 10.1128/iai.37.1.382-386.1982.
4
Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle.肺炎支原体感染：一种表面蛋白在附着细胞器中的作用
Science. 1982 Apr 16;216(4543):313-5. doi: 10.1126/science.6801766.
5
Isolation of binding sites to glycophorin from Mycoplasma pneumoniae membranes.从肺炎支原体膜中分离与血型糖蛋白的结合位点
Infect Immun. 1980 Dec;30(3):628-34. doi: 10.1128/iai.30.3.628-634.1980.
6
Inhibition of mycoplasma pneumoniae hemadsorption and adherence to respiratory epithelium by antibodies to a membrane protein.抗膜蛋白抗体对肺炎支原体血细胞吸附及黏附于呼吸道上皮的抑制作用。
Infect Immun. 1983 Mar;39(3):1180-6. doi: 10.1128/iai.39.3.1180-1186.1983.
7
Host discrimination of Mycoplasma pneumoniae proteinaceous immunogens.宿主对肺炎支原体蛋白质免疫原的识别。
J Exp Med. 1983 Feb 1;157(2):502-14. doi: 10.1084/jem.157.2.502.
8
An ELISA to detect monoclonal antibodies specific for lipid determinants of Mycoplasma pneumoniae.一种用于检测针对肺炎支原体脂质决定簇的单克隆抗体的酶联免疫吸附测定。
J Immunol Methods. 1983 Nov 11;64(1-2):165-78. doi: 10.1016/0022-1759(83)90395-2.
9
Tissue cultures and mycoplasmas.组织培养与支原体。
Tex Rep Biol Med. 1965 Jun;23:Suppl 1:285+.
10
Characteristics of virulent, attenuated, and avirulent Mycoplasma pneumoniae strains.有毒力、减毒和无毒力肺炎支原体菌株的特征。
J Bacteriol. 1969 Nov;100(2):1037-43. doi: 10.1128/jb.100.2.1037-1043.1969.