Organic solvents and temperature effects on desorption from immunoadsorbents. DNP-BSA anti-DNP as a model.
作者信息
Andersson K, Benyamin Y, Douzou P, Balny C
出版信息
J Immunol Methods. 1978;23(1-2):17-21. doi: 10.1016/0022-1759(78)90105-9.
PMID:29930
Abstract
Conditions for desorbing a DNP carrier from its immunoadsorbent were studied in various hydro-organic media at different pHs and temperatures, including the sub-zero range. It appeared that DNP--anti-DNP interaction is the result of a balance between various bonds and the best result (95% yield) was obtained in hydro-organic solvent at high pH and +30 degrees C.
摘要
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