Gibson J C, Rubinstein A, Ginsberg H N, Brown W V
Methods Enzymol. 1986;129:186-98. doi: 10.1016/0076-6879(86)29069-2.
These data suggest that subfractionation of lipoproteins by immunoaffinity chromatography according to apolipoprotein content provides a valuable method for physical isolation of intact lipoprotein subclasses. While this study has focused specifically on apoE-containing lipoproteins, the technique of immunoaffinity isolation clearly has wider application. It must be emphasized that antibodies differ with regard to affinity and avidity, and that both binding and elution conditions may need to be adjusted for each group of antibodies. Maintenance of physical integrity of lipoproteins thus isolated must also be evaluated independently for each antibody. Nevertheless, immunoaffinity isolation of apolipoprotein-specific subclasses offers a powerful tool for preparing lipoproteins which should reflect physical and/or metabolic properties conferred by the apolipoprotein of interest.
这些数据表明，根据载脂蛋白含量通过免疫亲和色谱法对脂蛋白进行亚分级分离，为完整脂蛋白亚类的物理分离提供了一种有价值的方法。虽然本研究专门聚焦于含载脂蛋白E的脂蛋白，但免疫亲和分离技术显然具有更广泛的应用。必须强调的是，抗体在亲和力和avidity方面存在差异，并且每组抗体可能都需要调整结合和洗脱条件。对于如此分离得到的脂蛋白，其物理完整性的维持也必须针对每种抗体独立进行评估。尽管如此，针对载脂蛋白特异性亚类的免疫亲和分离为制备脂蛋白提供了一个强大的工具，所制备的脂蛋白应能反映由目标载脂蛋白赋予的物理和/或代谢特性。
