Salk Institute for Biological Studies, La Jolla, California 92037, USA.
Biochemistry. 2011 Jul 26;50(29):6289-91. doi: 10.1021/bi2005448. Epub 2011 Jun 29.
In the budding yeast Saccharomyces cerevisiae, chromosome end protection is provided by a heterotrimeric complex composed of Cdc13 in association with the RPA-like proteins Stn1 and Ten1. We report here that the high affinity and specificity of the S. cerevisiae Cdc13 DNA binding domain for single-stranded telomeric DNA are not widely shared by other fungal Cdc13 proteins, suggesting that restriction of this complex to telomeres may be limited to the Saccharomyces clade. We propose that the evolutionarily conserved task of Stn1 and Ten1 (and their associated large subunit) is a genome-wide role in DNA replication rather than a telomere-dedicated activity.
在 budding 酵母 Saccharomyces cerevisiae 中，染色体末端保护由一个由 Cdc13 与 RPA 样蛋白 Stn1 和 Ten1 组成的异三聚体复合物提供。我们在此报告，S. cerevisiae Cdc13 DNA 结合域对单链端粒 DNA 的高亲和力和特异性并未广泛存在于其他真菌 Cdc13 蛋白中，这表明该复合物限制在端粒可能仅限于 Saccharomyces 分支。我们提出，Stn1 和 Ten1（及其相关的大亚基）的进化保守任务是在 DNA 复制中发挥全基因组作用，而不是专门针对端粒的活动。
