Department of Molecular Medicine, Laboratory of Virology, Sapienza University of Rome, Italy.
Naturamla S.r.l., Rome, Italy.
New Microbiol. 2023 May;46(2):161-169.
Ultraviolet-C (UVC) has been used to cause virus inactivation. The virucidal activity of three UV light lamps [UVC high frequencies (HF), UVC+B LED and UVC+A LED] was evaluated against the enveloped feline coronavirus (FCoVII), a surrogate model of SARS-CoV-2, the enveloped vesicular stomatitis virus (VSV), and the naked encephalomyocarditis virus (EMCV). Virucidal assays were performed at different time points of UV-light exposure (i.e., 5, 30 minutes and 1, 6, and 8 hours), placing each virus 180 cm below the perpendicular irradiation of the lamp and 1 and 2 meters from the perpendicular axis. We found that the UVC HF lamp had virucidal effects (≥96.8% of virus inactivation) against FCoVII, VSV and EMCV after 5 minutes of irradiation at each distance analyzed. Moreover, the UVC+B LED lamp had the highest inhibitory effects on FCoVII and VSV infectivity (≥99% of virus inactivation) when these viruses were settled below the perpendicular axis of the lamp for 5 minutes. Conversely, the UVC+A LED lamp was the least effective, achieving ≥85.9% inactivation of enveloped RNA viruses after 8 hours of UV exposure. Overall, UV light lamps, and in particular UVC HF and UVC+B LED ones, had a rapid and strong virucidal activity against distinct RNA viruses, including coronaviruses.
紫外线-C（UVC）已被用于使病毒失活。评估了三种紫外灯[UVC 高频（HF）、UVC+B LED 和 UVC+A LED]对包膜猫冠状病毒（FCoVII）、SARS-CoV-2 的替代模型、包膜水疱性口炎病毒（VSV）和裸露的脑心肌炎病毒（EMCV）的杀病毒活性。在不同的紫外线照射时间点（即 5、30 分钟以及 1、6 和 8 小时）进行杀病毒测定，将每种病毒置于灯的垂直照射下方 180 厘米处，以及距离垂直轴 1 和 2 米处。我们发现，UVC HF 灯在每个分析距离下照射 5 分钟后，对 FCoVII、VSV 和 EMCV 具有杀病毒作用（≥96.8%的病毒失活）。此外，当这些病毒位于灯的垂直轴下方 5 分钟时，UVC+B LED 灯对 FCoVII 和 VSV 感染性具有最高的抑制作用（≥99%的病毒失活）。相反，UVC+A LED 灯的效果最差，经过 8 小时的紫外线照射后，包膜 RNA 病毒的失活率≥85.9%。总体而言，紫外灯，特别是 UVC HF 和 UVC+B LED 灯，对不同的 RNA 病毒，包括冠状病毒，具有快速而强大的杀病毒活性。
