生物印迹脂肪酶催化合成富含二十二碳六烯酸的中长链结构脂用于婴儿配方奶粉。
Bioimprinted lipase-catalyzed synthesis of medium- and long-chain structured lipids rich in docosahexaenoic acid for infant formula.
机构信息
State Key Laboratory of Food Science and Resources, National Engineering Research Center for Functional Food, National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, China.
State Key Laboratory of Food Science and Resources, National Engineering Research Center for Functional Food, National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, China.
出版信息
Food Chem. 2023 Oct 30;424:136450. doi: 10.1016/j.foodchem.2023.136450. Epub 2023 May 24.
Medium- and long-chain structured lipids (MLSLs) rich in docosahexaenoic acid (DHA) were obtained in shorter reaction time by acidolysis of single-cell oil (DHASCO) from Schizochytrium sp. with caprylic acid (CA) using a lipase bioimprinted with fatty acids as a catalyst. The conditions for preparation of the bioimprinted lipase for the acidolysis reaction were firstly optimized and the activity of the obtained lipase was 2.17 times higher than that of the non-bioimprinted. The bioimprinted lipase was then used as a catalyst and the reaction conditions were optimized. Under the optimal conditions, the equilibrium could be achieved in 4 h, and the total and sn-1,3 CA contents in the product were 29.18% and 42.34%, respectively, and the total and sn-2 DHA contents were 46.26% and 70.12%, respectively. Such MLSLs rich in sn-1,3 CA and sn-2 DHA are beneficial for DHA absorption, and thus have potential for use in infant formula.
富含二十二碳六烯酸（DHA）的中长链结构脂（MLSL）可通过用脂肪酸印迹脂肪酶作为催化剂，使来自裂殖壶菌的单细胞油（DHASCO）与辛酸（CA）进行酸解反应，在更短的反应时间内获得。首先优化了用于酸解反应的生物印迹脂肪酶的制备条件，所得脂肪酶的活性比非生物印迹的高 2.17 倍。然后，将生物印迹脂肪酶用作催化剂并优化了反应条件。在最佳条件下，4 小时即可达到平衡，产物中的总 CA 和 sn-1,3 CA 含量分别为 29.18%和 42.34%，总 DHA 和 sn-2 DHA 含量分别为 46.26%和 70.12%。这种富含 sn-1,3 CA 和 sn-2 DHA 的 MLSL 有利于 DHA 的吸收，因此在婴儿配方奶粉中有潜在的应用前景。
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