Erzincan University Faculty of Medicine, Department of Medical Microbiology, Erzincan, Türkiye
University of Health Sciences Türkiye, Van Training and Research Hospital, Clinic of Infectious Diseases and Clinical Microbiology, Van, Türkiye
Turkiye Parazitol Derg. 2023 Jun 29;47(2):71-77. doi: 10.4274/tpd.galenos.2023.74936.
In this study, it was aimed to investigate the physical and chemical properties of different spring waters and parasitic factors with different methods.
This study was carried out on 69 water samples collected from different spring waters in and around Iğdır Province in April and June 2021. The samples were analyzed by native-Lugol, modified acid-fast staining, and nested polymerase chain reaction (nPCR). In addition, altitude (meter) and pressure (mmHg) measurements were made at the point where the water samples were taken.
One or more parasites were detected in 27.5% of the 69 water samples examined. Only was found in 13% of the samples, only spp. in 10.1%, only spp. in 1.4%, only and spp. in 1.4%, only , spp., and spp. in 1.4%. Only spp. cyst (4.3%) was detected by the direct examination method. While and spp. oocysts were detected in 8.7% and 7.2% of the samples by the modified acid-fast staining method, was detected in 15.9% and spp. was detected in 11.6% of the samples by nPCR. When the and spp. positivity rates were compared according to the characteristics of the water, there was no statistical difference between the altitude, salinity, pH, mmHg, and temperature (kelvin) values, but a significant correlation was found between the amount of dissolved oxygen and spp. positivity (p=0.047).
spp., , and are important waterborne pathogens that can cause epidemics. It is our belief that in order to reduce the risk of contamination of these parasitic factors with spring waters, public awareness should be raised, infrastructures should be improved, and new water treatment techniques, such as ultraviolet, ozonation and monitoring systems, should be used.
本研究旨在采用不同方法研究不同泉水的物理和化学性质及寄生虫因素。
本研究于 2021 年 4 月和 6 月在伊格尔迪尔省及其周边地区的 69 个不同泉水采集水样，采用 native-Lugol、改良酸性抗酸染色和巢式聚合酶链反应（nPCR）进行分析。此外，在采集水样的地点测量海拔（米）和压力（mmHg）。
在所检查的 69 个水样中，有 27.5%的水样检测到一种或多种寄生虫。在 13%的样本中仅发现 ，在 10.1%的样本中仅发现 spp.，在 1.4%的样本中仅发现 spp.，在 1.4%的样本中仅发现 和 spp.，在 1.4%的样本中仅发现 、 spp.和 spp.。仅通过直接检查法检测到 spp.包囊（4.3%）。改良酸性抗酸染色法检测到 8.7%和 7.2%的样本中 和 spp.卵囊，nPCR 检测到 15.9%和 11.6%的样本中 。根据水样特征比较 和 spp.阳性率，海拔、盐度、pH、mmHg 和温度（开尔文）值之间无统计学差异，但发现溶解氧与 spp.阳性率之间存在显著相关性（p=0.047）。
spp.、 、和 是重要的水源性寄生虫病原体，可引起流行病。我们认为，为了降低这些寄生虫因素污染泉水的风险，应提高公众意识，改善基础设施，并采用新的水处理技术，如紫外线、臭氧和监测系统。
