Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Department of Oncology, Shenzhen Hospital, Southern Medical University, Zhengzhou, China.
Biotechnol Genet Eng Rev. 2024 Dec;40(4):4769-4783. doi: 10.1080/02648725.2023.2216528. Epub 2023 May 30.
Colorectal cancer (CRC) is one of the most prevalent malignant tumors in the world. Research on long noncoding RNAs (LncRNAs) may illuminate tumorigenesis and progression of CRC.
We screened long non-coding RNA LINC00460 as a new candidate, which promoted the development of CRC in two independent datasets (GSE39582 and GSE21510) from the Gene Expression Omnibus (GEO). In 98 CRC tissues, expression levels of LINC00460 were significantly increased in cancerous tissues compared to paired adjacent normal tissues ( < 0.001). In addition, in the most common CRC cell lines. LINC00460 expression was up-regulated compared to normal human intestinal epithelial cell line NCM460. siRNA was transfected into CRC cell lines. LINC00460 knockdown reduced cell invasion ability and did not affect cell proliferation. The association between LINC00460 expression and clinical pathological features and prognosis were also analyzed.
This increased expression was found to significantly correlate with lymph node metastasis ( = 0.002), distant metastasis ( = 0.045) and TNM stage ( < 0.001); but not related to age, gender, location of tumor, and histological grade. The overall survival (OS) in CRC patients with overexpression of LINC00460 was inferior to that with low expression ( = 0.0167). Multivariate Cox regression analyses indicated that LINC00460 expression, as well as TNM stage was an independent prognostic risk factor for patients with CRC.
These results showed that a higher expression level of LINC00460 might play an oncogenic role in colorectal cancer invasion and metastasis. It also proved that LINC00460 might be used as a potential diagnostic and prognostic biomarker in CRC patients.
结直肠癌（CRC）是世界上最常见的恶性肿瘤之一。对长链非编码 RNA（LncRNAs）的研究可能阐明 CRC 的发生和发展。
我们筛选了长链非编码 RNA LINC00460 作为一个新的候选物，在来自基因表达综合数据库（GEO）的两个独立数据集（GSE39582 和 GSE21510）中，该基因促进了 CRC 的发展。在 98 例 CRC 组织中，癌组织中 LINC00460 的表达水平明显高于配对的相邻正常组织（<0.001）。此外，在最常见的 CRC 细胞系中，与正常的人肠上皮细胞系 NCM460 相比，LINC00460 的表达上调。将 siRNA 转染到 CRC 细胞系中，LINC00460 敲低降低了细胞侵袭能力，但不影响细胞增殖。还分析了 LINC00460 表达与临床病理特征和预后的关系。
发现这种表达增加与淋巴结转移（=0.002）、远处转移（=0.045）和 TNM 分期（<0.001）显著相关；但与年龄、性别、肿瘤位置和组织学分级无关。LINC00460 高表达的 CRC 患者的总生存期（OS）劣于低表达的患者（=0.0167）。多因素 Cox 回归分析表明，LINC00460 表达以及 TNM 分期是 CRC 患者的独立预后危险因素。
这些结果表明，LINC00460 的高表达水平可能在结直肠癌的侵袭和转移中发挥致癌作用。还证明 LINC00460 可能作为 CRC 患者潜在的诊断和预后生物标志物。
