College of Life Science, South-Central MinZu University, Wuhan, China.
College of Basic Medical Sciences, Shanxi University of Traditional Chinese Medicine, Jinzhong, China.
Can J Microbiol. 2023 Aug 1;69(8):321-327. doi: 10.1139/cjm-2022-0171. Epub 2023 May 30.
Endophytic bacteria play crucial roles in the growth and bioactive compound synthesis of host plants. In this study, the composition and diversity of endophytic bacteria in the roots, stems, and leaves from 3-year-old artificially cultivated were investigated using Illumina HiSeq sequencing technology. Total effective reads were assigned to 936 operational taxonomic units (OTUs), belonging to 12 phyla and 289 genera. A total of 28, 3, and 2 OTUs were exclusive to the roots, stems, and leaves, respectively. The bacterial richness and diversity in the roots were significantly lower than those in the leaves and stems. The dominant genera with significant distribution differences among these plant tissue samples were , and . Furthermore, the differences in the bacterial communities associated with these plant tissue samples were visualized using principal coordinate analysis and cluster pedigree diagrams. Linear discriminant analysis effect size explained statistically significant differences among the endophytic bacterial microbiota in these plant tissue samples. Overall, this study provides new insights into the diversity and distribution patterns of endophytic bacteria in the different tissues of .
内生细菌在宿主植物的生长和生物活性化合物合成中发挥着关键作用。本研究采用 Illumina HiSeq 测序技术，研究了 3 年生人工栽培的内生细菌在根、茎和叶中的组成和多样性。总有效读数被分配到 936 个操作分类单元（OTUs），属于 12 个门和 289 个属。共有 28、3 和 2 个 OTUs 分别仅存在于根、茎和叶中。根中的细菌丰富度和多样性明显低于叶和茎。在这些植物组织样本中具有显著分布差异的优势属为、和。此外，使用主坐标分析和聚类谱系图可视化了与这些植物组织样本相关的细菌群落的差异。线性判别分析效应大小解释了这些植物组织样本中内生细菌微生物群之间的统计学显著差异。总的来说，本研究为不同组织中的内生细菌多样性和分布模式提供了新的见解。
