College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China.
Laboratory of Useful Organisms, Research-Development Institute for Plant Protection, Ion Ionescu de la Brad no. 8 Blvd., 013813 Bucharest, Romania.
Molecules. 2021 Feb 8;26(4):892. doi: 10.3390/molecules26040892.
As the population ages globally, there seem to be more people with Alzheimer's disease. Unfortunately, there is currently no specific treatment for the disease. At present, Huperzine A (HupA) is one of the best drugs used for the treatment of Alzheimer's disease and has been used in clinical trials for several years in China. HupA was first separated from , a traditional medicinal herb that is used to cure fever, contusions, strains, hematuria, schizophrenia, and snakebite for several hundreds of years in China, and has been confirmed to have acetylcholinesterase inhibitory activity. With the very slow growth of , resources are becoming too scarce to meet the need for clinical treatment. Some endophytic fungal strains that produce HupA were isolated from in previous studies. In this article, the diversity of the endophytic fungal community within was observed and the relevance to the production of HupA by the host plant was further analyzed. A total of 1167 strains were obtained from the leaves of followed by the stems (1045) and roots (824). The richness as well as diversity of endophytic fungi within the leaf and stem were higher than in the root. The endophytic fungal community was similar within stems as well as in leaves at all taxonomic levels. The 11 genera (, , , , , , , , , , and ) were positively correlated with HupA content. The correlation index of with HupA contents displayed the highest value (CI = 0.92), whereas showed the lowest value (CI = 0.02). Through electrospray ionization mass spectrometry (ESI-MS), it was confirmed that the HS7-1 strain could produce HupA and the total alkaloid concentration was 3.7 ug/g. This study will enable us to screen and isolate the strain that can produce HupA and to figure out the correlation between endophytic fungal diversity with HupA content in different plant organs. This can provide new insights into the screening of strains that can produce HupA more effectively.
随着全球人口老龄化，似乎有更多的人患有阿尔茨海默病。不幸的是，目前该病尚无特定的治疗方法。目前，石杉碱甲（HupA）是治疗阿尔茨海默病的最佳药物之一，已在中国进行了数年的临床试验。HupA 最初是从中国传统草药千层塔中分离出来的，千层塔在中国已有数百年的历史，用于治疗发热、跌打损伤、血尿、精神分裂症和蛇咬伤，并已被证实具有乙酰胆碱酯酶抑制活性。由于千层塔的生长非常缓慢，资源变得过于稀缺，无法满足临床治疗的需求。在之前的研究中，从千层塔中分离出了一些产生 HupA 的内生真菌菌株。在本文中，观察了千层塔内生真菌群落的多样性，并进一步分析了其与宿主植物产生 HupA 的相关性。从千层塔的叶片中获得了 1167 株内生真菌，随后是茎（1045 株）和根（824 株）。叶片和茎内内生真菌的丰富度和多样性均高于根。在所有分类水平上，茎内和叶内的内生真菌群落都很相似。11 个属（、、、、、、、、和）与 HupA 含量呈正相关。与 HupA 含量的相关指数最高（CI = 0.92），而最低（CI = 0.02）。通过电喷雾电离质谱（ESI-MS），证实 HS7-1 菌株可以产生 HupA，总生物碱浓度为 3.7 ug/g。本研究将使我们能够筛选和分离能够产生 HupA 的菌株，并了解不同植物器官中内生真菌多样性与 HupA 含量的相关性。这可以为更有效地筛选能够产生 HupA 的菌株提供新的见解。
