雷公藤甲素甲醇提取物通过抗炎、抗氧化、5-α 还原酶缓解对雄性大鼠丙泊酚诱导的生殖毒性。
Rhus tripartite methanolic extract alleviates propylparaben-induced reproductive toxicity via anti-inflammatory, antioxidant, 5-α reductase in male rats.
机构信息
Biochemistry Department, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi Arabia.
Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
出版信息
Environ Sci Pollut Res Int. 2023 Jul;30(31):76969-76976. doi: 10.1007/s11356-023-27802-8. Epub 2023 May 30.
Evidence showed that herbal medicine could be beneficial for protection against diseases that may be exist in consequence of exposure to environmental toxicants. Propylparaben (PrP) is used as preservative in food, pharmaceuticals, and cosmetics. It is classified as one of endocrine disruptive chemicals (EDCs). This study evaluated the protective effect of Rhus tripartita methanolic extract (RTME) against reproductive toxicity induced by PrP in male rats. A total of 60 Wister albino rats were divided into four groups (15 rats for each group). Group I (control): rats received the vehicle (DMSO), group II: normal rats received RTME (10 mg/kg/day), group III: rats received PrP (10 mg/kg/day), and group IV: rats received PrP (10 mg/kg/day) and RTME (10 mg/kg/day) for 4 weeks. At the end of experiment, levels of testosterone, dihydrotestosterone (DHT), and 5α-reductase were analyzed in sera. Data obtained showed a significant reduction in the levels of testosterone, dihydrotestosterone (DHT), and 5α- reductase in rats given PrP versus control (p < 0.001) and RTME treatment improved these parameters but not returned to normal. Data obtained showed a significant elevation in levels of IL-6 and TNF-α in the testis of rats given PrP versus control (p < 0.001), these inflammatory mediators were significant reduced in rats treated with RTME compared with untreated rats (p < 0.001). There was a positive correlation between level of DHT and antioxidant enzymes activities (r = 0.56). A significant elevation in the levels of MDA with reduction in the activities of GST, GSPx, SOD, and catalase (p < 0.001) in rat testicular tissues of PrP group versus control (p < 0.001) was found. Treatment with RTME significantly reduced the levels of MDA and enhanced activities of GST, GSPx, SOD, and catalase (p < 0.001) compared to untreated group (p < 0.001). In conclusion, the active ingredient components of RTME abrogate the toxicity of PrP by exhibiting antioxidative and anti-inflammatory effects, enhancing 5-α reductase with improved hormonal status against PrP- induced testicular damage. Toxicity of propylparaben, and effect of Rhus tripartita methanolic extract.
证据表明，草药可能有助于预防因接触环境毒物而导致的疾病。对羟基苯甲酸丙酯（PrP）被用作食品、药品和化妆品的防腐剂。它被归类为内分泌干扰化学物质（EDCs）之一。本研究评估了 Rhus tripartita 甲醇提取物（RTME）对雄性大鼠 PrP 诱导的生殖毒性的保护作用。总共 60 只 Wister 白化大鼠被分为四组（每组 15 只）。第 I 组（对照组）：大鼠给予载体（DMSO），第 II 组：正常大鼠给予 RTME（10mg/kg/天），第 III 组：大鼠给予 PrP（10mg/kg/天），第 IV 组：大鼠给予 PrP（10mg/kg/天）和 RTME（10mg/kg/天），共 4 周。实验结束时，分析血清中的睾酮、二氢睾酮（DHT）和 5α-还原酶水平。结果表明，与对照组相比，给予 PrP 的大鼠的睾酮、二氢睾酮（DHT）和 5α-还原酶水平显著降低（p<0.001），而 RTME 治疗可改善这些参数，但不能恢复正常。结果表明，与对照组相比，给予 PrP 的大鼠睾丸中白细胞介素-6 和肿瘤坏死因子-α的水平显著升高（p<0.001），与未治疗组相比，用 RTME 治疗的大鼠这些炎症介质显著降低（p<0.001）。DHT 水平与抗氧化酶活性呈正相关（r=0.56）。与对照组相比，PrP 组大鼠睾丸组织中 MDA 水平升高，GST、GSPx、SOD 和过氧化氢酶活性降低（p<0.001）。与未治疗组相比，用 RTME 治疗可显著降低 MDA 水平，增强 GST、GSPx、SOD 和过氧化氢酶活性（p<0.001）。结论：RTME 的活性成分通过发挥抗氧化和抗炎作用，增强 5-α 还原酶，改善激素状态，拮抗 PrP 诱导的睾丸损伤，减轻 PrP 的毒性。对羟基苯甲酸丙酯的毒性和 Rhus tripartita 甲醇提取物的作用。
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