Diaz-Quevedo Clavel, Ramírez García Alonso, Torres Bernal Lenin, Cáceres Coral Jorge, Ampuero Trigoso Gustavo, Saucedo-Uriarte José Américo
Estación Experimental Agraria San Ramón, Instituto Nacional de Innovación Agraria, Yurimaguas 16501, Peru.
Facultad de Zootecnia, Agronomía Ciencias Biológicas y Acuicultura, Universidad Nacional Autónoma de Alto Amazonas, Yurimaguas 16501, Peru.
Vet Med Int. 2023 May 18;2023:9942021. doi: 10.1155/2023/9942021. eCollection 2023.
The objective of the research was to evaluate three synchronization protocols for fixed-time artificial insemination (FTAI) in with crossbred cows. Forty-five cows of 5 ± 1.6 years were selected. The Jsynch treatment consisted of the application of an intravaginal device plus benzoate; on day 8, the device was removed and PGF2, estradiol cypionate plus eCG was applied, and the FTAI was performed at 48-52 hours after. Conventional treatment consisted of the application of an intravaginal device plus benzoate; on day 8, the device was removed and PGF2 plus eCG was injected, and on day 9, estradiol benzoate was administered, and FTAI was performed at 30 hours. The Ovsynch treatment consisted of the application of GnRH, and on day 8, PGF2 was applied, on day 9, GnRH was applied again, and on day 10, FTAI plus GnRH was applied. The diagnosis of pregnancy was determined by transrectal ultrasound 45 days after the FTAI. The Jsynch (39.1%) and conventional (39.1%) treatment showed higher pregnancy compared to the Ovsynch treatment (21.7%) of pregnancy. The presence of crystalline mucus at the time of the FTAI was significantly associated with pregnancy. The results of this investigation indicate that it is possible to obtain acceptable pregnancy rates with the FTAI in with crossbred milk-producing cows, and treatments with progesterone-releasing devices plus eCG can improve the reproductive performance of cows.
本研究的目的是评估三种定时人工授精（FTAI）同步方案在杂交奶牛中的效果。选取了45头年龄为5±1.6岁的奶牛。Jsynch处理组包括放置阴道内装置并注射苯甲酸盐；在第8天取出装置，注射PGF2、环丙孕酮和eCG，在48 - 52小时后进行FTAI。传统处理组包括放置阴道内装置并注射苯甲酸盐；在第8天取出装置并注射PGF2和eCG，在第9天注射苯甲酸雌二醇，在30小时后进行FTAI。Ovsynch处理组包括注射GnRH，在第8天注射PGF2，在第9天再次注射GnRH，在第10天进行FTAI并注射GnRH。在FTAI后45天通过直肠超声诊断妊娠情况。Jsynch处理组（39.1%）和传统处理组（39.1%）的妊娠率高于Ovsynch处理组（21.7%）。FTAI时出现结晶状黏液与妊娠显著相关。本研究结果表明，在杂交产奶奶牛中采用FTAI可以获得可接受的妊娠率，使用释放孕酮的装置加eCG的处理可以提高奶牛的繁殖性能。
