García-Diéguez Laura, Diaz-Tang Gabriela, Marin Meneses Estefania, Cruise Vanessa, Barraza Ivana, Craddock Travis J A, Smith Robert P
Department of Biological Sciences, Halmos College of Arts and Science, Nova Southeastern University, Fort Lauderdale FL 33314, USA.
Clinical Systems Biology Group, Institute for Neuro-Immune Medicine, Nova Southeastern University, Fort Lauderdale FL 33314, USA.
iScience. 2023 May 11;26(6):106843. doi: 10.1016/j.isci.2023.106843. eCollection 2023 Jun 16.
uses quorum sensing to regulate the expression of virulence factors. In static environments, spatial structures, such as biofilms, can increase the expression of these virulence factors. However, in natural settings, biofilms are exposed to physical forces that disrupt spatial structure, which may affect the expression of virulence factors regulated by quorum sensing. We show that periodically disturbing biofilms composed of using a physical force reduces the expression of quorum sensing-regulated virulence factors. At an intermediate disturbance frequency, the expression of virulence factors in the , , and regulons is reduced. Mathematical modeling suggests that perturbation of the receptor is critical for this reduction. Removing the receptor enhances the reduction in the expression of virulence factors as a result of disturbance. Our results allow identification of environments where virulence is reduced and implicate the receptor as having a buffering role against disturbance.
利用群体感应来调节毒力因子的表达。在静态环境中，诸如生物膜等空间结构可增加这些毒力因子的表达。然而，在自然环境中，生物膜会受到破坏空间结构的物理力影响，这可能会影响由群体感应调节的毒力因子的表达。我们表明，使用物理力周期性地扰动由……组成的生物膜会降低群体感应调节的毒力因子的表达。在中等扰动频率下，……、……和……操纵子中毒力因子的表达会降低。数学模型表明，……受体的扰动对于这种降低至关重要。去除……受体可增强扰动导致的毒力因子表达的降低。我们的结果有助于确定毒力降低的环境，并表明……受体对干扰具有缓冲作用。
