Williams B G, Blattner F R
J Virol. 1979 Feb;29(2):555-75. doi: 10.1128/JVI.29.2.555-575.1979.
Twenty hybrid lambda phages especially designed for molecular cloning have been constructed and named Charon phages. These phages differ in the ranges of sizes of DNA fragments that may be inserted, by the selections and screens which may be used to isolate and detect the incorporation of cloned fragments, by the way transcription of the cloned fragment may be controlled, by the different restriction enzymes that can be used for cloning, by the phage immunities that may be employed for controlling replication and transcription, and by the biological safety features that they contain. The crosses used to produce the vectors are described, and their genealogy is discussed. The structure of each vector has been verified by genetic tests, by DNA length determinations, by electron micrographic analysis of DNA heteroduplexes, and by gel electrophoresis of restriction enzyme digests. In the course of these constructions, a new EcoRI site was found in a derivative of lambda Aam32Bam1 which maps very near the left cohesive end of lambda.
已经构建了20种专门用于分子克隆的杂交λ噬菌体，并将其命名为Charon噬菌体。这些噬菌体在可插入的DNA片段大小范围、用于分离和检测克隆片段掺入的选择和筛选方法、控制克隆片段转录的方式、可用于克隆的不同限制酶、可用于控制复制和转录的噬菌体免疫性以及它们所包含的生物安全特性等方面存在差异。描述了用于产生载体的杂交过程，并讨论了它们的谱系。每个载体的结构已通过遗传测试、DNA长度测定、DNA异源双链体的电子显微镜分析以及限制酶消化产物的凝胶电泳进行了验证。在这些构建过程中，在λ Aam32Bam1的一个衍生物中发现了一个新的EcoRI位点，该位点位于λ的左粘性末端附近。
