卡戎噬菌体：用于DNA克隆的λ噬菌体更安全的衍生物。

Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.

作者信息

Blattner F R, Williams B G, Blechl A E, Denniston-Thompson K, Faber H E, Furlong L, Grunwald D J, Kiefer D O, Moore D D, Schumm J W, Sheldon E L, Smithies O

出版信息

Science. 1977 Apr 8;196(4286):161-9. doi: 10.1126/science.847462.

DOI:10.1126/science.847462

PMID:847462

Abstract

The Charon lambda bacteriophages have been developed as vectors for cloning. Their construction incorporates mutations that make them simple to use and also greatly increases their safety for the biological containment of cloned recombinant DNA. Three of the Charon vector phages, 3A, 4A, and 16A, have been certified for use as EK2 vector-host systems, when propagated in bulk in a special bacterial host, DP50SupF. We present here some of the data on which the safety of these systems was evaluated. DNA fragments ranging in size from 0 to 2.2 X 10(4) base pairs can be cloned in these EK2 Charon phages.

摘要

卡戎λ噬菌体已被开发用作克隆载体。它们的构建引入了一些突变，这些突变使其易于使用，同时也大大提高了其在生物安全方面对克隆重组DNA的容纳能力。三种卡戎载体噬菌体，即3A、4A和16A，当在特殊细菌宿主DP50SupF中大量繁殖时，已被认证可用作EK2载体-宿主系统。我们在此展示一些用于评估这些系统安全性的数据。大小从0到2.2×10⁴碱基对的DNA片段可克隆到这些EK2卡戎噬菌体中。