通过文库筛选发现的 pH 感应超分子荧光探针。
pH-sensing supramolecular fluorescent probes discovered by library screening.
机构信息
Department of Chemistry, Fudan University, Shanghai, 200438, China.
School of Life Science & Institute of Advanced Studies, Taizhou University, Jiaojiang, 318000, Zhejiang, China.
出版信息
Talanta. 2023 Oct 1;263:124716. doi: 10.1016/j.talanta.2023.124716. Epub 2023 May 26.
A new design concept for pH-sensing supramolecular fluorescent probes is reported. Supramolecular fluorescent pH probes based on pro-guest are designed and prepared. Pro-guests are designed to degrade under acidic condition and convert to competitive guests to displace encapsulated dyes, which leads to a significant enhancement in fluorescence intensity. A library of potential fluorescent pH probes is generated and screened to discover workable probes. These probes are capable of detecting the acidic pH in solution phase. We confirm that these supramolecular probes could detect the acidic environment in endosomal compartments in live cells.
本文报道了一种用于 pH 传感的超分子荧光探针的全新设计理念。设计并制备了基于主体分子的超分子荧光 pH 探针。主体分子在酸性条件下发生降解，并转化为竞争性客体分子，从而取代包封的染料，导致荧光强度显著增强。生成并筛选了一个潜在荧光 pH 探针库，以发现可行的探针。这些探针能够检测溶液相中酸性 pH 值。我们证实，这些超分子探针能够检测活细胞内内涵体区室中的酸性环境。
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