School of Pharmaceutical Engineering & Institute for Advanced Studies, Taizhou University, Jiaojiang 318000, Zhejiang, China; Department of Chemistry, Fudan University, Shanghai 200433, China.
School of Pharmaceutical Engineering & Institute for Advanced Studies, Taizhou University, Jiaojiang 318000, Zhejiang, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 15;303:123167. doi: 10.1016/j.saa.2023.123167. Epub 2023 Jul 19.
A new ratiometric fluorescent probe is designed and prepared based on the concept of supramolecular encapsulation and dye competition. This supramolecular probe is based on two commercially-available dyes, one common guest and a simple-to-synthesize host. Fluorescence spectroscopy confirms that the supramolecular probe is capable of detecting thiols quantitatively with a broad linear region in phosphate buffered saline or fetal bovine serum. Mechanistic study shows a reaction between thiol specie and the guest to alter the distribution of encapsulated dyes. The supramolecular probes are demonstrated to quantitatively detect extracellular biological thiols by plate reader, which shows it keeps its effectiveness in complex buffered systems.
一种基于超分子包合和染料竞争概念设计和制备的新型比率荧光探针。这种超分子探针基于两种市售染料、一种常见客体和一种简单合成的主体。荧光光谱证实，该超分子探针能够在磷酸盐缓冲液或胎牛血清中定量检测巯基，具有较宽的线性范围。机理研究表明，巯基物种与客体之间的反应改变了包封染料的分布。通过平板读数器证明，该超分子探针可以定量检测细胞外生物巯基，表明它在复杂缓冲体系中仍然有效。
