Jiang Siyang, Yang Jingyu, Ling Li, Wang Shuyi, Ma Da
School of Pharmaceutical and Materials Engineering & Institute for Advanced Studies, Taizhou University, Jiaojiang, Zhejiang 318000, China.
Department of Chemistry, Fudan University, Shanghai 200433, China.
Anal Chem. 2022 Apr 12;94(14):5634-5641. doi: 10.1021/acs.analchem.1c05647. Epub 2022 Mar 31.
Supramolecular fluorescent probes for the detection of reactive oxygen species (ROSs) are designed based on a pro-guest strategy. Nine commercially available fluorescent dyes, six host molecules, and a pro-guest are used to rapidly generate a library of 54 potential supramolecular probes. These potential supramolecular probes are screened in a high-throughput fashion using a plate reader to discover seven "hits" or workable probes. The mechanism is confirmed to be ROS-induced conversion from a low-binding-affinity pro-guest to a high-binding-affinity guest and the competitive displacement of the encapsulated fluorescent dye. The response to HO of four supramolecular probes is found to be concentration-dependent and may be used for quantitative analysis of HO. The supramolecular probe is selectively responsive toward other oxidative agents, such as NaClO and NaSO. The cell study shows that supramolecular probes are capable of detecting HO in human cancer cells (MCF-7 or HeLa).
基于主客体策略设计了用于检测活性氧（ROS）的超分子荧光探针。使用九种市售荧光染料、六种主体分子和一种前体客体快速生成了一个包含54种潜在超分子探针的文库。使用酶标仪以高通量方式对这些潜在的超分子探针进行筛选，以发现七种“命中”探针或可行探针。其作用机制被确认为是由ROS诱导的从前体客体的低结合亲和力向客体的高结合亲和力的转变以及被包封荧光染料的竞争性置换。发现四种超分子探针对HO的响应呈浓度依赖性，可用于HO的定量分析。该超分子探针对其他氧化剂如NaClO和NaSO具有选择性响应。细胞研究表明，超分子探针能够检测人癌细胞（MCF-7或HeLa）中的HO。
