College of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao, Shandong, 266590, PR China.
College of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao, Shandong, 266590, PR China.
Anal Chim Acta. 2023 Aug 1;1267:341386. doi: 10.1016/j.aca.2023.341386. Epub 2023 May 17.
Various nanomaterials with peroxidase activity (nanozyme) have been designed for bio catalysis and biosensing, however, most of them need further design and modification of probe molecules for the specific binding reaction with targets. This results in a decrease in catalysis activity and hinders them to be perfect alternatives to natural enzyme in biosensing. In this work, an enhanced nanozyme was synthesized by functionalizing natural microperoxidase-11 (MP-11) on a hybrid graphene oxide-gold (GO-Au) material. The designed nanozyme showed an enhanced catalysis activity and realized a robust and efficient colorimetric detection of cysteine based on specific binding reaction between active iron center from MP-11 and thiol in cysteine. The enhanced properties show promising applications of complex nanozyme and provides a great opportunity for developing efficient sensing systems.
各种具有过氧化物酶活性的纳米材料（纳米酶）已被设计用于生物催化和生物传感，然而，它们大多数需要进一步设计和修饰探针分子，以与目标进行特异性结合反应。这导致催化活性降低，阻碍了它们在生物传感中成为天然酶的完美替代品。在这项工作中，通过在混合氧化石墨烯-金（GO-Au）材料上功能化天然微过氧化物酶-11（MP-11），合成了一种增强型纳米酶。设计的纳米酶表现出增强的催化活性，并基于 MP-11 的活性铁中心与半胱氨酸中的巯基之间的特异性结合反应，实现了对半胱氨酸的稳健且高效的比色检测。增强的性能展示了复杂纳米酶的应用前景，并为开发高效传感系统提供了巨大机会。
