Ian Potter NanoBioSensing Facility, NanoBiotechnology Research Laboratory (NBRL), School of Science, RMIT University, GPO Box 2476, Melbourne, VIC 3000, Australia.
Division of Biological & Life Sciences, School of Arts and Sciences, Central Campus, Ahmedabad University, Navrangura, Ahmedabad 380009, Gujarat, India.
Biosens Bioelectron. 2018 Jul 1;110:8-15. doi: 10.1016/j.bios.2018.03.025. Epub 2018 Mar 13.
Enzyme-mimicking catalytic nanoparticles, more commonly known as NanoZymes, have been at the forefront for the development of new sensing platforms for the detection of a range of molecules. Although solution-based NanoZymes have shown promise in glucose detection, the ability to immobilize NanoZymes on highly absorbent surfaces, particularly on free-standing substrates that can be feasibly exposed and removed from the reaction medium, can offer significant benefits for a range of biosensing and catalysis applications. This work, for the first time, shows the ability of Ag nanoparticles embedded within the 3D matrix of a cotton fabric to act as a free-standing peroxidase-mimic NanoZyme for the rapid detection of glucose in complex biological fluids such as urine. The use of cotton fabric as a template not only allows high number of catalytically active sites to participate in the enzyme-mimic catalytic reaction, the absorbent property of the cotton fibres also helps in rapid absorption of biological molecules such as glucose during the sensing event. This, in turn, brings the target molecule of interest in close proximity of the NanoZyme catalyst enabling accurate detection of glucose in urine. Additionally, the ability to extract the free-standing cotton fabric-supported NanoZyme following the reaction overcomes the issue of potential interference from colloidal nanoparticles during the assay. Based on these unique characteristics, nanostructured silver fabrics offer remarkable promise for the detection of glucose and other biomolecules in complex biological and environmental fluids.
酶模拟催化纳米粒子，通常称为纳米酶，一直处于开发新型传感平台以检测多种分子的前沿。虽然基于溶液的纳米酶在葡萄糖检测方面显示出了潜力，但将纳米酶固定在高吸收性表面上的能力，特别是在可以从反应介质中实际暴露和去除的独立基底上，可为各种生物传感和催化应用带来显著的优势。这项工作首次表明，嵌入棉织物 3D 基质中的银纳米粒子能够作为独立的过氧化物酶模拟纳米酶，用于快速检测尿液等复杂生物流体中的葡萄糖。使用棉织物作为模板不仅允许大量的催化活性位点参与酶模拟催化反应，棉纤维的吸水性还有助于在传感事件中快速吸收葡萄糖等生物分子。这反过来又使感兴趣的目标分子接近纳米酶催化剂，从而能够准确检测尿液中的葡萄糖。此外，在反应后提取独立的棉织物支撑的纳米酶的能力克服了在测定过程中胶体纳米粒子潜在干扰的问题。基于这些独特的特性，纳米结构银织物在复杂的生物和环境流体中检测葡萄糖和其他生物分子方面具有显著的前景。
