Histology and Embryology Laboratory, Medical School, Faculty of Health, Aristotle University of Thessaloniki, Thessaloniki, Greece.
School of Biology, Aristotle University of Thessaloniki, Thessaloniki, Greece.
J Matern Fetal Neonatal Med. 2023 Dec;36(1):2218523. doi: 10.1080/14767058.2023.2218523.
Unexplained recurrent pregnancy loss has been a a challenging research task to experts since there is no explicit pathophysiological mechanism and therefore, the treatment remains elusive. Immunological imbalance and morphological abnormalities are under investigation. This study aims to evaluate the implication of MMP-2, MMP-9, EGFR, and IL-8 in recurrent pregnancy loss cases.
MATERIALS & METHODS: The study was carried out through comparison among two groups; the unexplained miscarriage group which consisted of 22 women, and the control group consisted of 18 women, who had electively terminated their pregnancies. Both groups were in the first trimester of gestation. The specimens included the trophoblast, decidua basalis, and decidua parietalis. The study was conducted immunohistochemical methods. Antibodies were used against MMP-2, MMP-9, EGFR, and IL-8. The results were presented at a contingency table and were statistically analyzed with the Chi-Square Test (X).
There were remarkable disparities in some cases in the comparison of the two groups. MMP-9 was detected significantly high in recurrent pregnancy loss (RPL) cases, both on trophoblastic and decidual specimens (-value < .00001), MMP-2 displayed no difference among the two groups (mild to moderate detection on trophoblast and almost negative on decidual tissues). EGFR was highly detected in trophoblastic tissue (-value = .014). IL-8 detection was particularly different in both trophoblast and decidua parietalis of the two groups (-value < .01).
The study revealed both morphological and immunological dysregulations that might participate in the RPL pathogenesis.
不明原因的复发性流产一直是专家们面临的一项极具挑战性的研究课题，因为目前尚无明确的病理生理机制，因此治疗方法仍难以捉摸。免疫失衡和形态异常正在研究中。本研究旨在评估 MMP-2、MMP-9、EGFR 和 IL-8 在复发性流产病例中的意义。
该研究通过两组之间的比较进行；不明原因流产组 22 例，对照组 18 例，均为选择性终止妊娠。两组均处于妊娠早期。标本包括滋养层、基底蜕膜和壁蜕膜。采用免疫组织化学方法。使用针对 MMP-2、MMP-9、EGFR 和 IL-8 的抗体。结果以列联表形式呈现，并采用卡方检验（X）进行统计学分析。
两组比较时，某些病例存在显著差异。MMP-9 在复发性流产（RPL）病例中检测到明显升高，在滋养层和蜕膜标本中均如此（-值<.00001），MMP-2 在两组之间无差异（滋养层呈轻度至中度检出，蜕膜组织几乎为阴性）。EGFR 在滋养层组织中高度检出（-值=0.014）。IL-8 在两组的滋养层和壁蜕膜中的检出均有明显差异（-值<.01）。
本研究揭示了可能参与 RPL 发病机制的形态和免疫失调。
